HAMAP

Accession	MF_00801
Dates	24-NOV-2004 (Created) 11-OCT-2011 (Last updated, Version 21)

Data class

Protein

Predictors

HAMAP; MF_00801; [distribution of match scores in UniProtKB];[seed alignment for MF_00801]

Names

Endonuclease_5

Identifier

NFI

Protein name

RecName: Full=Endonuclease V; EC=3.1.21.7; AltName: Full=Deoxyinosine 3'endonuclease; AltName: Full=Deoxyribonuclease V; Short=DNase V;

Gene name

nfi

FUNCTION: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Has a wide substrate spectrum. In addition to deoxyinosine-containing DNA, the enzyme cleaves DNA containing urea residues, AP sites, base mismatches, insertion/deletion mismatches, flaps, and pseudo-Y structures. Participates in the excision repair of hypoxanthine and xanthine (deaminated adenine and guanine) in DNA. It thereby reduces the mutagenic effects of nitrous acid by attacking lesions caused by nitrosative deamination (By similarity).

FUNCTION: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair (By similarity).

CATALYTIC ACTIVITY: Endonucleolytic cleavage at apurinic or apyrimidinic sites to products with a 5'-phosphate.

COFACTOR: Magnesium (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the endonuclease V family.

Pfam	PF04493; Endonuclease_5; 1;
TIGRFAMs	TIGR00589; Ogt; 1;

Cytoplasm, DNA damage, DNA repair, Endonuclease, Hydrolase, Magnesium, Metal-binding, Nuclease.

GO:0000287; Molecular function: magnesium ion binding.
GO:0043737; Molecular function: deoxyribonuclease V activity.
GO:0006281; Biological process: DNA repair.
GO:0005737; Cellular component: cytoplasm.

From: NFI_THEMA (Q9X2H9)
Key		From		To		Description		Condition		FTGroup
METAL		43		43		Magnesium (By similarity)		D
METAL		110		110		Magnesium (By similarity)		[DEN]
SITE		80		80		Interaction with target DNA (By similarity)		Y

Size range:	191-255 amino acids
Related UniRules:	None
Template:	P68739 (NFI_ECOLI); O30108 (NFI_ARCFU); Q9X2H9 (NFI_THEMA): [Recover all]
Scope:	Bacteria Archaea
Fusion:	Nter: <Methyltransferase>; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	Some archaea are binfunctional and contains a N-terminal methyltransferase domain.