HAMAP rule MF_00847
General rule information
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| Accession | MF_00847 |
| Dates | 26-NOV-2019 (Created)
1-JUN-2023 (Last updated, Version 8) |
| Name | EttA |
| Scope(s) |
Bacteria |
| Template(s) | P0A9W3 (ETTA_ECOLI); [ Recover all ] |
| Triggered by |
HAMAP; MF_00847 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | ETTA |
| Protein name | RecName: Full=Energy-dependent translational throttle protein EttA; EC=3.6.1.-; AltName: Full=Translational regulatory factor EttA; |
| Gene name | Name=ettA; |
Comments
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| FUNCTION | A translation factor that gates the progression of the 70S ribosomal initiation complex (IC, containing tRNA(fMet) in the P-site) into the translation elongation cycle by using a mechanism sensitive to the ATP/ADP ratio. Binds to the 70S ribosome E-site where it modulates the state of the translating ribosome during subunit translocation. ATP hydrolysis probably frees it from the ribosome, which can enter the elongation phase. |
| CATALYTIC ACTIVITY | Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; |
| SUBUNIT | Monomer. Probably contacts ribosomal proteins L1, L5, L33 and S7, the 16S and 23S rRNA and the P-site containing tRNA(fMet). |
| SUBCELLULAR LOCATION | Cytoplasm. Note=Associates with ribosomes and polysomes. |
| DOMAIN | The arm domain is inserted in the first ABC transporter domain. Probably contacts ribosomal protein L1. |
| DOMAIN | The P-site tRNA interaction motif (PtIM domain) probably interacts with the P-site tRNA(fMet) as well as the 23S rRNA. |
| SIMILARITY | Belongs to the ABC transporter superfamily. ABCF family. Translational throttle EttA subfamily. |
Keywords
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| ATP-binding |
| Cytoplasm |
| Hydrolase |
| Nucleotide-binding |
| Protein biosynthesis |
| Repeat |
| RNA-binding |
| rRNA-binding |
| Translation regulation |
| tRNA-binding |
Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0016887; Molecular function:ATP hydrolysis activity |
| GO:0005737; Cellular component:cytoplasm |
| GO:0019843; Molecular function:rRNA binding |
| GO:0000049; Molecular function:tRNA binding |
| GO:0043022; Molecular function:ribosome binding |
| GO:0045900; Biological process:negative regulation of translational elongation |
Cross-references
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| PROSITE | PS00211; ABC_TRANSPORTER_1; 1; |
| PROSITE | PS50893; ABC_TRANSPORTER_2; 2; |
| Pfam | PF00005; ABC_tran; 2; |
| Pfam | PF12848; ABC_tran_Xtn; 1; |
| NCBIfam | TIGR03719; ABC_ABC_ChvD; 1; |
Features
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| From: ETTA_ECOLI (P0A9W3) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DOMAIN | 6 | 259 | /note="ABC transporter 1" | |||||||||
| DOMAIN | 324 | 550 | /note="ABC transporter 2" | |||||||||
| BINDING | 39 | 46 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" /ligand_label="1" |
G-x-N-G-[ATV]-G-K-[ST] | ||||||||
| BINDING | 356 | 363 | /ligand="ATP" /ligand_id="ChEBI:CHEBI:30616" /ligand_label="2" |
G-x-N-G-[SICAV]-G-K-[TS] | ||||||||
| REGION | 95 | 139 | /note="Arm" | |||||||||
| REGION | 242 | 322 | /note="PtIM" | |||||||||
Additional information
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| Size range | 544-646 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |