HAMAP rule MF_00952
General rule information
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| Accession | MF_00952 |
| Dates | 22-MAR-2013 (Created)
10-OCT-2023 (Last updated, Version 11) |
| Name | Topoisom_1_prok |
| Scope(s) |
Bacteria Archaea |
| Template(s) | P06612 (TOP1_ECOLI); P9WG49 (TOP1_MYCTU); [ Recover all ] |
| Triggered by |
HAMAP; MF_00952 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | TOP1 |
| Protein name | RecName: Full=DNA topoisomerase 1; EC=5.6.2.1; AltName: Full=DNA topoisomerase I; |
| Gene name | Name=topA; |
Comments
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| FUNCTION | Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA- (5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. |
| CATALYTIC ACTIVITY | Reaction=ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.; EC=5.6.2.1; |
| SUBUNIT | Monomer. |
| SIMILARITY | Belongs to the type IA topoisomerase family. |
Keywords
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Gene Ontology
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| GO:0003917; Molecular function:DNA topoisomerase type I (single strand cut, ATP-independent) activity |
| GO:0006265; Biological process:DNA topological change |
Cross-references
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| PROSITE | PS00396; TOPO_IA_1; 1; |
| PROSITE | PS52039; TOPO_IA_2; 1; |
| PROSITE | PS50880; TOPRIM; 1; |
| Pfam | PF08272; Topo_Zn_Ribbon; 0-2; |
| Pfam | PF01131; Topoisom_bac; 1; |
| Pfam | PF01751; Toprim; 1; |
| Pfam | PF01396; zf-C4_Topoisom; 0-3; |
| PRINTS | PR00417; PRTPISMRASEI; 1; |
| NCBIfam | TIGR01051; topA_bact; 0-1; |
| NCBIfam | TIGR01057; topA_arch; 0-1; |
Features
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| From: TOP1_ECOLI (P06612) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| REGION | 192 | 197 | /note="Interaction with DNA" | S-x-G-R-V-Q | ||||||||
| ACT_SITE | 319 | 319 | /note="O-(5'-phospho-DNA)-tyrosine intermediate" | Y | ||||||||
| SITE | 33 | 33 | /note="Interaction with DNA" | H | ||||||||
| SITE | 168 | 168 | /note="Interaction with DNA" | R | ||||||||
| SITE | 169 | 169 | /note="Interaction with DNA" | R | ||||||||
| SITE | 172 | 172 | /note="Interaction with DNA" | D | ||||||||
| SITE | 177 | 177 | /note="Interaction with DNA" | Y | ||||||||
| SITE | 184 | 184 | /note="Interaction with DNA" | W | ||||||||
| SITE | 321 | 321 | /note="Interaction with DNA" | R | ||||||||
| SITE | 507 | 507 | /note="Interaction with DNA" | R | ||||||||
Additional information
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| Size range | 560-1100 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |