HAMAP rule MF_00978
General rule information
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| Accession | MF_00978 |
| Dates | 9-DEC-2013 (Created)
1-JUN-2023 (Last updated, Version 13) |
| Name | Bifunct_BirA |
| Scope(s) |
Bacteria |
| Template(s) | P06709 (BIRA_ECOLI); [ Recover all ] |
| Triggered by |
case c? <OC:Bacteria>
HAMAP; MF_00978 (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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| Identifier | BIRA |
| case <OC:Gammaproteobacteria> | |
| Protein name | RecName: Full=Bifunctional ligase/repressor BirA; AltName: Full=Biotin--[acetyl-CoA-carboxylase] ligase; EC=6.3.4.15; AltName: Full=Biotin--protein ligase; AltName: Full=Biotin-[acetyl-CoA carboxylase] synthetase; AltName: Full=Biotin operon repressor; |
| else | |
| Protein name | RecName: Full=Bifunctional ligase/repressor BirA; AltName: Full=Biotin--[acetyl-CoA-carboxylase] ligase; EC=6.3.4.15; AltName: Full=Biotin--protein ligase; AltName: Full=Biotin-[acetyl-CoA carboxylase] synthetase; |
| end case | |
| Gene name | Name=birA; |
Comments
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| case <OC:Gammaproteobacteria> | |
| FUNCTION | Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a biotin-operon repressor. In the presence of ATP, BirA activates biotin to form the BirA-biotinyl-5'-adenylate (BirA-bio-5'-AMP or holoBirA) complex. HoloBirA can either transfer the biotinyl moiety to the biotin carboxyl carrier protein (BCCP) subunit of acetyl-CoA carboxylase, or bind to the biotin operator site and inhibit transcription of the operon. |
| else | |
| FUNCTION | Acts both as a biotin--[acetyl-CoA-carboxylase] ligase and a repressor. |
| end case | |
| CATALYTIC ACTIVITY | Reaction=ATP + biotin + L-lysyl-[protein] = AMP + diphosphate + H(+) + N(6)-biotinyl-L-lysyl-[protein]; Xref=Rhea:RHEA:11756, Rhea:RHEA- COMP:9752, Rhea:RHEA-COMP:10505, ChEBI:CHEBI:15378, ChEBI:CHEBI:29969, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57586, ChEBI:CHEBI:83144, ChEBI:CHEBI:456215; EC=6.3.4.15; |
| SIMILARITY | Belongs to the biotin--protein ligase family. |
Keywords
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| ATP-binding |
| Biotin |
| DNA-binding |
| Ligase |
| Nucleotide-binding |
| Repressor |
| Transcription |
| Transcription regulation |
Gene Ontology
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| GO:0005524; Molecular function:ATP binding |
| GO:0004077; Molecular function:biotin-[acetyl-CoA-carboxylase] ligase activity |
| GO:0003677; Molecular function:DNA binding |
| GO:0006355; Biological process:regulation of DNA-templated transcription |
Cross-references
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| PROSITE | PS51733; BPL_LPL_CATALYTIC; 1; |
| Pfam | PF02237; BPL_C; 1; |
| Pfam | PF03099; BPL_LplA_LipB; 1; |
| Pfam | PF08279; HTH_11; 1; |
| NCBIfam | TIGR00121; birA_ligase; 1; |
| NCBIfam | TIGR00122; birA_repr_reg; 1; |
Features
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| From: BIRA_ECOLI (P06709) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| DNA_BIND | 22 | 41 | /note="H-T-H motif" | |||||||||
| BINDING | 89 | 91 | /ligand="biotin" /ligand_id="ChEBI:CHEBI:57586" |
[ST]-[ST]-N | ||||||||
| BINDING | 116 | 118 | /ligand="biotin" /ligand_id="ChEBI:CHEBI:57586" |
R-G-R | ||||||||
| BINDING | 112 | 112 | /ligand="biotin" /ligand_id="ChEBI:CHEBI:57586" |
Q | ||||||||
| BINDING | 183 | 183 | /ligand="biotin" /ligand_id="ChEBI:CHEBI:57586" |
K | ||||||||
Additional information
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| Size range | 280-400 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |