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HAMAP rule MF_00983

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_00983
Accession MF_00983
Dates 7-JAN-2014 (Created)
13-JAN-2025 (Last updated, Version 7)
Name PriA
Scope(s) Bacteria
Template(s) P17888 (PRIA_ECOLI); P94461 (PRIA_BACSU); P0DXX8 (PRIA_DEIRA); [ Recover all ]
Triggered by HAMAP; MF_00983 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier PRIA
case not <Feature:PS51192> and not <Feature:PS51194>
Protein name RecName: Full=Probable replication restart primosome protein PriA;
AltName: Full=Putative ATP-dependent DNA helicase PriA;
else
Protein name RecName: Full=ATP-dependent DNA helicase PriA;
                 EC=5.6.2.4;
AltName: Full=DNA 3'-5' helicase PriA;
AltName: Full=Replication restart primosome protein PriA;
end case
Gene name Name=priA;

Comments [?]

FUNCTIONInitiates the restart of stalled replication forks. Recognizes and binds the arrested nascent DNA chain at stalled replication forks. Promotes assembly of the primosome and loading of the major replicative helicase onto DNA.
case <Feature:PS51192> or <Feature:PS51194>
CATALYTIC ACTIVITY Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by translocating in the 3'-5' direction.; EC=5.6.2.4;
CATALYTIC ACTIVITY Reaction=ATP + H2O = ADP + phosphate + H(+); Xref=Rhea:RHEA:13065, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
end case
case <FTTag:zinc1> and <FTTag:zinc2>
COFACTOR Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 2 zinc ions per subunit.;
end case
SUBUNITComponent of the replication restart primosome.
SIMILARITYBelongs to the helicase family. PriA subfamily.
case not <Feature:PS51192> and not <Feature:PS51194>
CAUTIONAs this protein does not have any detectable helicase domains, it probably does not have helicase activity.
end case

Keywords [?]

ATP-binding
DNA replication
DNA-binding
case <Feature:PS51192> or <Feature:PS51194>
Helicase
Hydrolase
Isomerase
end case
Nucleotide-binding
Primosome
case <FTTag:zinc1> and <FTTag:zinc2>
Metal-binding
Zinc
end case

Gene Ontology [?]

case <Feature:PS51192> or <Feature:PS51194>
GO:0003678; Molecular function:DNA helicase activity
end case
GO:0005524; Molecular function:ATP binding
GO:0003677; Molecular function:DNA binding
case <FTTag:zinc1> and <FTTag:zinc2>
GO:0008270; Molecular function:zinc ion binding
end case
GO:0006260; Biological process:DNA replication
GO:1990077; Cellular component:primosome complex

Cross-references [?]

PROSITE PS51192; HELICASE_ATP_BIND_1; 0-1;
PROSITE PS51194; HELICASE_CTER; 0-1;
Pfam PF00270; DEAD; 1;
Pfam PF00271; Helicase_C; 1;
NCBIfam TIGR00595; priA; 1;

Features [?]

From: PRIA_ECOLI (P17888)
Key From To Description Tag Condition FTGroup
BINDING 436 436 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"		 zinc1 C
BINDING 439 439 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"		 zinc1 C
BINDING 445 445 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"		 zinc2 C
BINDING 448 448 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"		 zinc2 C
BINDING 463 463 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"		 zinc2 C
BINDING 466 466 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="2"		 zinc2 C
BINDING 476 476 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"		 zinc1 C
BINDING 479 479 /ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"
/ligand_label="1"		 zinc1 C

Additional information [?]

Size range 535-950 amino acids
Related rules None
Fusion Nter: None Cter: None



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