HAMAP

Accession	MF_01033
Dates	1-OCT-2002 (Created) 11-OCT-2011 (Last updated, Version 32)

Data class

Protein

Predictors

HAMAP; MF_01033; [distribution of match scores in UniProtKB];[seed alignment for MF_01033]

Names

LeuB_type1

Identifier

LEU3

Protein name

RecName: Full=3-isopropylmalate dehydrogenase; EC=1.1.1.85; AltName: Full=3-IPM-DH; AltName: Full=Beta-IPM dehydrogenase; Short=IMDH;

Gene name

leuB

FUNCTION: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate (By similarity).

CATALYTIC ACTIVITY: (2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.

COFACTOR: Binds 1 magnesium or manganese ion per subunit (By similarity).

PATHWAY: Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.

SUBUNIT: Homodimer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Pfam	PF00180; Iso_dh; 1;
TIGRFAMs	TIGR00169; LeuB; 1;
PROSITE	PS00470; IDH_IMDH; 1;

Cytoplasm, Amino-acid biosynthesis, Branched-chain amino acid biosynthesis, Leucine biosynthesis, Magnesium, Manganese, Metal-binding, NAD, Oxidoreductase.

GO:0003862; Molecular function: 3-isopropylmalate dehydrogenase activity.
GO:0009098; Biological process: leucine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

From: LEU3_THET8 (Q5SIY4)
Key		From		To		Description		Condition		FTGroup
NP_BIND (Optional)		74		87		NAD (By similarity)		G-[PYSAEKRT]-[KQHTAER]-x(0,3)-[WYV]-x(6,8)-P-E
NP_BIND (Optional)		274		286		NAD (By similarity)		G-S-A-P-[DNT]-[IL]-x(4,5)-[AV]-N
METAL		217		217		Magnesium or manganese (By similarity)		D		1
METAL		241		241		Magnesium or manganese (By similarity)		D		1
METAL		245		245		Magnesium or manganese (By similarity)		D		1
BINDING		94		94		Substrate (By similarity)		R
BINDING		104		104		Substrate (By similarity)		R
BINDING		132		132		Substrate (By similarity)		R
BINDING		217		217		Substrate (By similarity)		D
SITE		139		139		Important for catalysis (By similarity)		Y
SITE		185		185		Important for catalysis (By similarity)		K

Size range:	344-381 amino acids
Related UniRules:	MF_01035 (LEU3)
Template:	P30125 (LEU3_ECOLI); P37412 (LEU3_SALTY); P61495 (LEU3_THETH); Q56268 (LEU3_THIFE); Q5SIY4 (LEU3_THET8): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	in BORBR, BRAJA, DECAR
Plasmid encoded:	in BUCAI, BUCAP, BUCDN, BUCML, BUCRP, BUCTS, BUCUA, BUCUD, BUCUE, BUCUH, BUCUM, BUCUN, BUCUO, BUCUS
Comments:	See MF_01035 for Actinomycetales leuB. Archaeal leuB are divergent and are not included in the alignment.