To improve security and privacy, we are moving our web pages and services from HTTP to HTTPS.
To give users of web services time to transition to HTTPS, we will support separate HTTP and HTTPS services until the end of 2017.
From January 2018 most HTTP traffic will be automatically redirected to HTTPS. [more...]
View this page in https
Annotation rule MF_01035
Send feedback

General rule information [?]

Accession MF_01035
Dates 1-OCT-2002 (Created)
22-JUN-2015 (Last updated, Version 29)
Name LeuB_type2
Scope Bacteria; Actinobacteria
Templates P30125 (LEU3_ECOLI); P37412 (LEU3_SALTY); P61495 (LEU3_THETH); Q56268 (LEU3_ACIFR); Q5SIY4 (LEU3_THET8): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=3-isopropylmalate dehydrogenase;
AltName: Full=3-IPM-DH;
AltName: Full=Beta-IPM dehydrogenase;
Gene name

Comments [?]

Function Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate.
Catalytic activity (2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
Cofactor Mg(2+)
Note: Binds 1 Mg(2+) or Mn(2+) ion per subunit.
Pathway Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit Homodimer.
Subcellular location Cytoplasm.
Similarity Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003862; Molecular function: 3-isopropylmalate dehydrogenase activity.
GO:0009098; Biological process: leucine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00180; Iso_dh; 1;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     271     283       NAD     G-S-A-P-D-I-x(5)-A-[DN]  
METAL     211     211       Magnesium or manganese     D   1
METAL     235     235       Magnesium or manganese     D   1
METAL     239     239       Magnesium or manganese     D   1
BINDING     87     87       Substrate     R  
BINDING     97     97       Substrate     R  
BINDING     121     121       Substrate     R  
BINDING     211     211       Substrate     D  
SITE     128     128       Important for catalysis     Y  
SITE     178     178       Important for catalysis     K  

Additional information [?]

Size range 335-357 amino acids
Related rules MF_01033 (LEU3)
Fusion None
Comments See MF_01033 for other leuB