HAMAP annotation rule MF_01035
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Accession MF_01035
Dates 1-OCT-2002 (Created)
17-AUG-2010 (Last updated, Version 25)
Name LeuB_type2
Scope Bacteria; Actinobacteridae
Triggered by



Identifier
LEU3
Protein name
RecName: Full=3-isopropylmalate dehydrogenase;
EC=1.1.1.85;
AltName: Full=3-IPM-DH;
AltName: Full=Beta-IPM dehydrogenase;
Short=IMDH;
Gene name
leuB


FUNCTION Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate (By similarity).
CATALYTIC ACTIVITY (2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
COFACTOR Binds 1 magnesium or manganese ion per subunit (By similarity).
PATHWAY Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate; step 3/4.
SUBUNIT Homodimer (By similarity).
SUBCELLULAR LOCATION Cytoplasm (By similarity).
SIMILARITY Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily.


Cytoplasm
Amino-acid biosynthesis
Branched-chain amino acid biosynthesis
Leucine biosynthesis
Magnesium
Manganese
Metal-binding
NAD
Oxidoreductase


GO:0003862; Molecular function: 3-isopropylmalate dehydrogenase activity.
GO:0009098; Biological process: leucine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.


Pfam PF00180; Iso_dh; 1;
PROSITE PS00470; IDH_IMDH; 1;


From: LEU3_MYCTU (P95313)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     271     283       NAD (By similarity)     G-S-A-P-D-I-x(5)-A-[DN]  
METAL     211     211       Magnesium or manganese (By similarity)     D   1
METAL     235     235       Magnesium or manganese (By similarity)     D   1
METAL     239     239       Magnesium or manganese (By similarity)     D   1
BINDING     87     87       Substrate (By similarity)     R  
BINDING     97     97       Substrate (By similarity)     R  
BINDING     121     121       Substrate (By similarity)     R  
BINDING     211     211       Substrate (By similarity)     D  
SITE     128     128       Important for catalysis (By similarity)     Y  
SITE     178     178       Important for catalysis (By similarity)     K  


Size range: 335-357 amino acids
Related Rules: MF_01033 (LEU3)
Templates: P30125 (LEU3_ECOLI); P37412 (LEU3_SALTY); P61495 (LEU3_THETH); Q56268 (LEU3_THIFE); Q5SIY4 (LEU3_THET8): [Recover all]
Fusion: None
Comments: See MF_01033 for other leuB


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