Annotation rule MF_01035
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General rule information [?]

Accession MF_01035
Dates 1-OCT-2002 (Created)
25-FEB-2014 (Last updated, Version 26)
Name LeuB_type2
Scope Bacteria; Actinobacteridae
Templates P30125 (LEU3_ECOLI); P37412 (LEU3_SALTY); P61495 (LEU3_THETH); Q56268 (LEU3_ACIFR); Q5SIY4 (LEU3_THET8): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=3-isopropylmalate dehydrogenase;
AltName: Full=3-IPM-DH;
AltName: Full=Beta-IPM dehydrogenase;
Gene name

Comments [?]

Function Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate (By similarity).
Catalytic activity (2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
Cofactor Binds 1 magnesium or manganese ion per subunit (By similarity).
Pathway Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate; step 3/4.
Subunit Homodimer (By similarity).
Subcellular location Cytoplasm (By similarity).
Similarity Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 2 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003862; Molecular function: 3-isopropylmalate dehydrogenase activity.
GO:0009098; Biological process: leucine biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00180; Iso_dh; 1;

Features [?]

Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     271     283       NAD (By similarity)     G-S-A-P-D-I-x(5)-A-[DN]  
METAL     211     211       Magnesium or manganese (By similarity)     D   1
METAL     235     235       Magnesium or manganese (By similarity)     D   1
METAL     239     239       Magnesium or manganese (By similarity)     D   1
BINDING     87     87       Substrate (By similarity)     R  
BINDING     97     97       Substrate (By similarity)     R  
BINDING     121     121       Substrate (By similarity)     R  
BINDING     211     211       Substrate (By similarity)     D  
SITE     128     128       Important for catalysis (By similarity)     Y  
SITE     178     178       Important for catalysis (By similarity)     K  

Additional information [?]

Size range 335-357 amino acids
Related rules MF_01033 (LEU3)
Fusion None
Comments See MF_01033 for other leuB