HAMAP

Accession	MF_01037
Dates	28-NOV-2002 (Created) 11-OCT-2011 (Last updated, Version 21)

Data class

Protein

Predictors

HAMAP; MF_01037; [distribution of match scores in UniProtKB];[seed alignment for MF_01037]

Names

TrmFO

Identifier

TRMFO

Protein name

RecName: Full=Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO; EC=2.1.1.74; AltName: Full=Folate-dependent tRNA (uracil-5-)-methyltransferase; AltName: Full=Folate-dependent tRNA(M-5-U54)-methyltransferase;

Gene name

trmFO

FUNCTION: Catalyzes the folate-dependent formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs (By similarity).

CATALYTIC ACTIVITY: 5,10-methylenetetrahydrofolate + tRNA containing uridine at position 54 + FADH(2) = tetrahydrofolate + tRNA containing ribothymidine at position 54 + FAD.

COFACTOR: FAD (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the MnmG family. TrmFO subfamily.

Pfam	PF01134; GIDA; 1;
TIGRFAMs	TIGR00137; Gid_trmFO; 1;
PROSITE	PS01280; GIDA_1; 1; PS01281; GIDA_2; 1;

Cytoplasm, FAD, Flavoprotein, Methyltransferase, Transferase, tRNA processing.

GO:0050660; Molecular function: flavin adenine dinucleotide binding.
GO:0009021; Molecular function: tRNA (uracil-5-)-methyltransferase activity.
GO:0008033; Biological process: tRNA processing.
GO:0005737; Cellular component: cytoplasm.

From: TRMFO_BACSU (P39815)
Key		From		To		Description		Condition		FTGroup
NP_BIND		10		15		FAD (By similarity)		G-x-G-[LIM]-[AS]-G

Size range:	364-482 amino acids
Related UniRules:	MF_00129 (MNMG)
Template:	P39815 (TRMFO_BACSU); Q9S449 (TRMFO_MYXXD): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	in MESFL, MYCCT, MYCMS
Plasmid encoded:	None