Annotation rule MF_01057
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General rule information [?]

Accession MF_01057
Dates 12-JUN-2003 (Created)
23-SEP-2013 (Last updated, Version 22)
Name tRNA_methyltr_TrmB
Scope Bacteria

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
TRMB
Protein name
RecName: Full=tRNA (guanine-N(7)-)-methyltransferase;
EC=2.1.1.33;
AltName: Full=tRNA (guanine(46)-N(7))-methyltransferase;
AltName: Full=tRNA(m7G46)-methyltransferase;
Gene name
trmB

Comments [?]

Function Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA (By similarity).
Catalytic activity S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.
Pathway tRNA modification; N(7)-methylguanine-tRNA biosynthesis.
case <OC:Enterobacteriaceae>
Subunit Monomer (By similarity).
end case
Similarity Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.

Keywords [?]


Gene Ontology [?]

GO:0008176; Molecular function: tRNA (guanine-N7-)-methyltransferase activity.
GO:0006400; Biological process: tRNA modification.

Cross-references [?]

PROSITE PS51625; SAM_MT_TRMB; 1;
Pfam PF02390; Methyltransf_4; 1;
TIGRFAMs TIGR00091; TIGR00091; 1;

Features [?]

From: TRMB_ECOLI (P0A8I5)
Key     From     To       Description   Tag   Condition   FTGroup
REGION (Optional)     150     155       Interaction with RNA (Potential)     [KR]-[KH]-[EN]-K-R-R  
REGION (Optional)     217     220       Substrate binding (Potential)     T-x-[YF]-E  
BINDING (Optional)     69     69       S-adenosyl-L-methionine (By similarity)     [DE]  
BINDING (Optional)     94     94       S-adenosyl-L-methionine (By similarity)     [DE]  
BINDING (Optional)     121     121       S-adenosyl-L-methionine (By similarity)     [DEN]  
BINDING (Optional)     144     144       S-adenosyl-L-methionine (By similarity)     [DN]  
BINDING (Optional)     148     148       Substrate (By similarity)     K  
BINDING (Optional)     180     180       Substrate (Potential)     D  

Additional information [?]

Size range 185-400 amino acids
Related rules None
Templates P0A8I5 (TRMB_ECOLI); O34522 (TRMB_BACSU); P67506 (TRMB_STRPN); O66479 (TRMB_AQUAE): [Recover all]
Fusion Nter: hemK, kdtA; Cter: None
Comments Fused to hemK in RICBR, RICCN, RICFE, RICPR and RICTY, and fused to kdtA in FUSNN; Unknown N-terminal domain in METPP; sequences not included in alignment and not taken into account in size range