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HAMAP rule MF_01080
General rule information
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| Accession | MF_01080 |
| Dates | 24-SEP-2003 (Created)
1-JUN-2023 (Last updated, Version 27) |
| Name | TruB_bact |
| Scope(s) |
Bacteria |
| Template(s) | P60340 (TRUB_ECOLI); P9WHP7 (TRUB_MYCTU); Q9WZW0 (TRUB_THEMA); [ Recover all ] |
| Triggered by |
HAMAP; MF_01080 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | TRUB |
| Protein name | RecName: Full=tRNA pseudouridine synthase B; EC=5.4.99.25; AltName: Full=tRNA pseudouridine(55) synthase; Short=Psi55 synthase; AltName: Full=tRNA pseudouridylate synthase; AltName: Full=tRNA-uridine isomerase; |
| Gene name | Name=truB; |
Comments
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| FUNCTION | Responsible for synthesis of pseudouridine from uracil-55 in the psi GC loop of transfer RNAs. |
| CATALYTIC ACTIVITY | Reaction=uridine(55) in tRNA = pseudouridine(55) in tRNA; Xref=Rhea:RHEA:42532, Rhea:RHEA-COMP:10101, Rhea:RHEA-COMP:10102, ChEBI:CHEBI:65314, ChEBI:CHEBI:65315; EC=5.4.99.25; |
| SIMILARITY | Belongs to the pseudouridine synthase TruB family. Type 1 subfamily. |
Keywords
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Gene Ontology
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| GO:0009982; Molecular function:pseudouridine synthase activity |
| GO:0031119; Biological process:tRNA pseudouridine synthesis |
Cross-references
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| Pfam | PF01509; TruB_N; 1; |
| Pfam | PF01472; PUA; 1; |
| NCBIfam | TIGR00431; TruB; 1; |
| PROSITE | PS50890; PUA; 0-1; |
Features
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| From: TRUB_ECOLI (P60340) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| ACT_SITE | 48 | 48 | /note="Nucleophile" | D | ||||||||
| case <OC:Enterobacterales> | ||||||||||||
| BINDING | 43 | 43 | /ligand="substrate" | H | ||||||||
| BINDING | 76 | 76 | /ligand="substrate" | Y | ||||||||
| BINDING | 179 | 179 | /ligand="substrate" | Y | ||||||||
| BINDING | 200 | 200 | /ligand="substrate" | L | ||||||||
| end case | ||||||||||||
Additional information
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| Size range | 218-387 amino acids |
| Related rules |
MF_01081 |
| Fusion | Nter: None Cter: None |