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HAMAP rule MF_01084
General rule information
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| Accession | MF_01084 |
| Dates | 3-OCT-2003 (Created)
28-JUL-2023 (Last updated, Version 25) |
| Name | Diphthine_synth |
| Scope(s) |
Archaea |
| Template(s) | O58456 (DPHB_PYRHO); Q9YDI2 (DPHB_AERPE); [ Recover all ] |
| Triggered by |
HAMAP; MF_01084 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | DPHB |
| Protein name | RecName: Full=Diphthine synthase; EC=2.1.1.98; AltName: Full=Diphthamide biosynthesis methyltransferase; |
| Gene name | Name=dphB; |
Comments
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| FUNCTION | S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis. |
| CATALYTIC ACTIVITY | Reaction=2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 3 S-adenosyl-L-methionine = diphthine- [translation elongation factor 2] + 3 H(+) + 3 S-adenosyl-L- homocysteine; Xref=Rhea:RHEA:36415, Rhea:RHEA-COMP:9749, Rhea:RHEA- COMP:10172, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:73995, ChEBI:CHEBI:82696; EC=2.1.1.98; |
| PATHWAY | Protein modification; peptidyl-diphthamide biosynthesis. |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the diphthine synthase family. |
Keywords
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Gene Ontology
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| GO:0004164; Molecular function:diphthine synthase activity |
| GO:0017183; Biological process:protein histidyl modification to diphthamide |
Cross-references
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| Pfam | PF00590; TP_methylase; 1; |
| NCBIfam | TIGR00522; Dph5; 1; |
| PIRSF | PIRSF036432; Diphthine_synth; 1; |
Features
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| From: DPHB_PYRHO (O58456) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 115 | 116 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[STN]-[IGVA] | ||||||||
| BINDING | 10 | 10 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[LI] | ||||||||
| BINDING | 87 | 87 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
D | ||||||||
| BINDING | 90 | 90 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[VALMTIG] | ||||||||
| BINDING | 166 | 166 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[LIV] | ||||||||
| BINDING | 209 | 209 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[ALVM] | ||||||||
| BINDING | 234 | 234 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[HYQ] | ||||||||
Additional information
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| Size range | 206-300 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |
| Comments | Possible wrong C-terminal sequence in THEVO |