HAMAP

Accession	MF_01102
Dates	16-JUL-2002 (Created) 19-AUG-2011 (Last updated, Version 23)

Data class

Protein

Predictors

HAMAP; MF_01102; [distribution of match scores in UniProtKB];[seed alignment for MF_01102]

Names

MnmC

Identifier

MNMC

Protein name

RecName: Full=tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC; Short=tRNA mnm(5)s(2)U biosynthesis bifunctional protein; RecName: Full=tRNA (mnm(5)s(2)U34)-methyltransferase; EC=2.1.1.61; RecName: Full=FAD-dependent cmnm(5)s(2)U34 oxidoreductase; EC=1.5.-.-;

Gene name

mnmC

FUNCTION: Catalyzes the last two steps in the biosynthesis of 5-methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl group from S-adenosyl-L-methionine to nm(5)s(2)U34, to form mnm(5)s(2)U34 (By similarity).

CATALYTIC ACTIVITY: S-adenosyl-L-methionine + tRNA = S-adenosyl-L-homocysteine + tRNA containing 5-methylaminomethyl-2-thiouridylate.

COFACTOR: FAD (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (Potential).

SIMILARITY: In the N-terminal section; belongs to the methyltransferase superfamily. tRNA (mnm(5)s(2)U34)-methyltransferase family.

SIMILARITY: In the C-terminal section; belongs to the DAO family.

Pfam	PF01266; DAO; 1; PF05430; DUF752; 1;
TIGRFAMs	TIGR03197; MnmC_Cterm; 1;

Cytoplasm, FAD, Flavoprotein, Methyltransferase, Multifunctional enzyme, Oxidoreductase, S-adenosyl-L-methionine, Transferase, tRNA processing.

GO:0050660; Molecular function: flavin adenine dinucleotide binding.
GO:0016491; Molecular function: oxidoreductase activity.
GO:0008757; Molecular function: S-adenosylmethionine-dependent methyltransferase activity.
GO:0002097; Biological process: tRNA wobble base modification.
GO:0005737; Cellular component: cytoplasm.

From: MNMC_ECOLI (P77182)
Key		From		To		Description		Condition		FTGroup
REGION		Nter		245		tRNA (mnm(5)s(2)U34)-methyltransferase
REGION		270		Cter		FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Size range:	585-754 amino acids
Related UniRules:	None
Template:	P77182 (MNMC_ECOLI)
Scope:	Bacteria; Proteobacteria Bacteria; Spirochaetes
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None