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HAMAP rule MF_01106

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General rule information [?]

PURL https://purl.expasy.org/hamap/rule/MF_01106
Accession MF_01106
Dates 28-MAR-2003 (Created)
9-DEC-2024 (Last updated, Version 41)
Name ArgJ
Scope(s) Bacteria
Archaea
Template(s) Q07908 (ARGJ_GEOSE); P9WPZ3 (ARGJ_MYCTU); Q9Z4S1 (ARGJ_THENN); P0DJQ5 (GNAT2_STRCL); Q9K8V3 (ARGJ_HALH5); Q57645 (ARGJ_METJA); [ Recover all ]
Triggered by
case c? <OC:Bacteria> or <OC:Archaea>
HAMAP; MF_01106 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri>
Identifier ARGJ
Protein name RecName: Full=Glutamate N-acetyltransferase;
                 EC=2.3.1.35;
AltName: Full=Ornithine acetyltransferase;
                 Short=OATase;
AltName: Full=Ornithine transacetylase;
                 Contains:
RecName: Full=Glutamate N-acetyltransferase alpha chain;
                 Contains:
RecName: Full=Glutamate N-acetyltransferase beta chain;
Gene name Name=argJ;
else
Identifier ARGJ
Protein name RecName: Full=Arginine biosynthesis bifunctional protein ArgJ;
                 Includes:
RecName: Full=Glutamate N-acetyltransferase;
                 EC=2.3.1.35;
AltName: Full=Ornithine acetyltransferase;
                 Short=OATase;
AltName: Full=Ornithine transacetylase;
                 Includes:
RecName: Full=Amino-acid acetyltransferase;
                 EC=2.3.1.1;
AltName: Full=N-acetylglutamate synthase;
                 Short=AGSase;
                 Contains:
RecName: Full=Arginine biosynthesis bifunctional protein ArgJ alpha chain;
                 Contains:
RecName: Full=Arginine biosynthesis bifunctional protein ArgJ beta chain;
Gene name Name=argJ;
end case

Comments [?]

case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri>
FUNCTIONCatalyzes the transfer of the acetyl group from N(2)- acetylornithine to glutamate, forming N-acetylglutamate and L- ornithine.
CATALYTIC ACTIVITY Reaction=N(2)-acetyl-L-ornithine + L-glutamate = N-acetyl-L-glutamate + L-ornithine; Xref=Rhea:RHEA:15349, ChEBI:CHEBI:29985, ChEBI:CHEBI:44337, ChEBI:CHEBI:46911, ChEBI:CHEBI:57805; EC=2.3.1.35;
PATHWAYAmino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.
else
FUNCTIONCatalyzes two activities which are involved in the cyclic version of arginine biosynthesis: the synthesis of N-acetylglutamate from glutamate and acetyl-CoA as the acetyl donor, and of ornithine by transacetylation between N(2)-acetylornithine and glutamate.
CATALYTIC ACTIVITY Reaction=N(2)-acetyl-L-ornithine + L-glutamate = N-acetyl-L-glutamate + L-ornithine; Xref=Rhea:RHEA:15349, ChEBI:CHEBI:29985, ChEBI:CHEBI:44337, ChEBI:CHEBI:46911, ChEBI:CHEBI:57805; EC=2.3.1.35;
CATALYTIC ACTIVITY Reaction=L-glutamate + acetyl-CoA = N-acetyl-L-glutamate + CoA + H(+); Xref=Rhea:RHEA:24292, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:44337, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288; EC=2.3.1.1;
PATHWAYAmino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1.
PATHWAYAmino-acid biosynthesis; L-arginine biosynthesis; N(2)-acetyl- L-ornithine from L-glutamate: step 1/4.
end case
SUBUNITHeterotetramer of two alpha and two beta chains.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the ArgJ family.

Keywords [?]

Cytoplasm
Amino-acid biosynthesis
Arginine biosynthesis
Autocatalytic cleavage
case not <OC:Methanobacteria> and not <OC:Methanococci> and not <OC:Methanomicrobia> and not <OC:Methanopyri>
Multifunctional enzyme
end case
Transferase
Acyltransferase

Gene Ontology [?]

case <OCellular component:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri>
GO:0004358; Molecular function:glutamate N-acetyltransferase activity
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0004358; Molecular function:glutamate N-acetyltransferase activity
GO:0004042; Molecular function:L-glutamate N-acetyltransferase activity
end case
GO:0006526; Biological process:L-arginine biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

Pfam PF01960; ArgJ; 1;
NCBIfam TIGR00120; ArgJ; 1;

Features [?]

From: ARGJ_MYCTU (P9WPZ3)
Key From To Description Tag Condition FTGroup
case <OC:Methanobacteria> or <OC:Methanococci> or <OC:Methanomicrobia> or <OC:Methanopyri>
CHAIN Nter 199 /note="Glutamate N-acetyltransferase alpha chain"
CHAIN 200 Cter /note="Glutamate N-acetyltransferase beta chain"
else
CHAIN Nter 199 /note="Arginine biosynthesis bifunctional protein ArgJ alpha chain"
CHAIN 200 Cter /note="Arginine biosynthesis bifunctional protein ArgJ beta chain"
end case
ACT_SITE 200 200 /note="Nucleophile" [TS]
BINDING 166 166 /ligand="substrate" T
BINDING 189 189 /ligand="substrate" K
BINDING 200 200 /ligand="substrate" [TS]
BINDING 280 280 /ligand="substrate" E
BINDING 399 399 /ligand="substrate" N
BINDING 404 404 /ligand="substrate" [ST]
SITE 127 127 /note="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" T
SITE 128 128 /note="Involved in the stabilization of negative charge on the oxyanion by the formation of the oxyanion hole" G
SITE 199 200 /note="Cleavage; by autolysis" [AG]-[TS]

Additional information [?]

Size range 379-432 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Some archaea/bacteria possess a monofunctional ArgJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway.



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