HAMAP annotation rule MF_01109
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Accession MF_01109
Dates 11-JUL-2003 (Created)
11-OCT-2011 (Last updated, Version 29)
Name OTCase
Scope Bacteria
Archaea
Triggered by



Identifier
OTC
Protein name
RecName: Full=Ornithine carbamoyltransferase;
Short=OTCase;
EC=2.1.3.3;
case not <Property:ARGININE_BS>
Identifier
OTCC
Protein name
RecName: Full=Ornithine carbamoyltransferase, catabolic;
Short=OTCase;
EC=2.1.3.3;
end case
case <Property:ARGININE_BS> and <OC:Enterobacteriaceae>
Gene name
argI
end case
case <Property:ARGININE_BS> and not <OC:Enterobacteriaceae>
Gene name
argF
end case
case not <Property:ARGININE_BS>
Gene name
arcB
end case


CATALYTIC ACTIVITY Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.
case <Property:ARGININE_BS>
PATHWAY Amino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate; step 1/3.
end case
case not <Property:ARGININE_BS>
PATHWAY Amino-acid degradation; L-arginine degradation via ADI pathway; carbamoyl phosphate from L-arginine; step 2/2.
end case
SUBCELLULAR LOCATION Cytoplasm (By similarity).
SIMILARITY Belongs to the ATCase/OTCase family.


case <Property:ARGININE_BS>
Amino-acid biosynthesis
Arginine biosynthesis
end case
case not <Property:ARGININE_BS>
Arginine metabolism
end case
Cytoplasm
Transferase


GO:0004585; Molecular function: ornithine carbamoyltransferase activity.
case <Property:ARGININE_BS>
GO:0006526; Biological process: arginine biosynthetic process.
end case
case not <Property:ARGININE_BS>
GO:0019546; Biological process: arginine deiminase pathway.
end case
GO:0005737; Cellular component: cytoplasm.


Pfam PF00185; OTCace; 1;
PF02729; OTCace_N; 1;
PRINTS PR00100; AOTCASE; 1;
TIGRFAMs TIGR00658; Orni_carb_tr; 1;
PROSITE PS00097; CARBAMOYLTRANSFERASE; 1;


From: OTCC_PSEAE (P08308)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     57     61       Carbamoyl phosphate binding (By similarity)     S-T-R-T-R  
REGION     273     276       Ornithine binding (By similarity)     H-C-L-P  
BINDING     108     108       Carbamoyl phosphate (By similarity)     R  
BINDING     135     135       Carbamoyl phosphate (By similarity)     H  
SITE (Optional)     32     32       Important for structural integrity (By similarity)     K  
SITE (Optional)     148     148       Important for structural integrity (By similarity)     E  


Size range: 295-354 amino acids
Related Rules: MF_02102 (PTC (supersedes the current rule))
Template: P04391 (OTC1_ECOLI)
Fusion: None
Comments: OTCases can be involved in the biosynthesis of arginine and in its catabolism, by the arginine deiminase pathway. Some bacteria possess two distinct OTCases; some do not degrade arginine using this pathway and others do not synthesize arginine. As it is impossible to distinguish between anabolic and catabolic OTCases based on sequence similarity alone, if paralogs are found in a given genome, then one is probably the anabolic enzyme and the other the catabolic one, named arcB, which is usually located in the arcABC operon. E.coli K-12 carries two genes for anabolic OTCase, argF and argI; their products interact to form a family of four trimeric isoenzymes. Only argI can be found in E.coli B, E.coli W or in other enterobacteria.


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