HAMAP annotation rule MF_01113
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Accession MF_01113
Dates 7-JUN-2002 (Created)
6-JAN-2012 (Last updated, Version 30)
Name DNApol_IV
Scope Bacteria
Archaea
Triggered by



Identifier
DPO4
Protein name
RecName: Full=DNA polymerase IV;
Short=Pol IV;
EC=2.7.7.7;
case <OC:Bacteria>
Gene name
dinB
end case
case <OC:Archaea>
Gene name
dbh
end case


case <OC:Bacteria>
FUNCTION Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from PolIII (By similarity).
end case
case <OC:Archaea>
FUNCTION Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by PolIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis (By similarity).
end case
CATALYTIC ACTIVITY Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
COFACTOR Binds 2 magnesium ions per subunit (By similarity).
SUBUNIT Monomer (By similarity).
SUBCELLULAR LOCATION Cytoplasm (By similarity).
SIMILARITY Belongs to the DNA polymerase type-Y family.
case <Feature:PS50173>
SIMILARITY Contains 1 umuC domain.
end case


Cytoplasm
Transferase
Nucleotidyltransferase
DNA-directed DNA polymerase
DNA replication
DNA damage
DNA repair
DNA-binding
Mutator protein
Metal-binding
Magnesium


GO:0003677; Molecular function: DNA binding.
GO:0003887; Molecular function: DNA-directed DNA polymerase activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006261; Biological process: DNA-dependent DNA replication.
GO:0006281; Biological process: DNA repair.
GO:0005737; Cellular component: cytoplasm.


Pfam PF00817; IMS; 1;
PROSITE PS50173; UMUC; 1; trigger=PRU00216;


From: DPO4_SULSF (P96022)
Key     From     To       Description   Tag   Condition   FTGroup
ACT_SITE     106     106       By similarity     E  
METAL     7     7       Magnesium (By similarity)     D  
METAL     105     105       Magnesium (By similarity)     D  
SITE     12     12       Substrate discrimination (By similarity)        


Size range: 318-479 amino acids
Related Rules: None
Templates: Q47155 (DPO4_ECOLI); P96022 (DPO4_SULSF); Q97W02 (DPO4_SULSO): [Recover all]
Fusion: None


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