HAMAP

Accession	MF_01113
Dates	7-JUN-2002 (Created) 21-APR-2011 (Last updated, Version 29)

Data class

Protein

Predictors

HAMAP; MF_01113; [distribution of match scores in UniProtKB];[seed alignment for MF_01113]

Names

DNApol_IV

Identifier

DPO4

Protein name

RecName: Full=DNA polymerase IV; Short=Pol IV; EC=2.7.7.7;

Gene name

dinB

Gene name

dbh

FUNCTION: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis, in conjunction with the beta clamp from polIII (By similarity).

FUNCTION: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits no 3'-5' exonuclease (proofreading) activity. May be involved in translesional synthesis (By similarity).

CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).

COFACTOR: Binds 2 magnesium ions per subunit (By similarity).

SUBUNIT: Monomer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the DNA polymerase type-Y family.

SIMILARITY: Contains 1 umuC domain.

Pfam	PF00817; IMS; 1;
PROSITE	PS50173; UMUC; 1; trigger=PRU00216;

Cytoplasm, Transferase, Nucleotidyltransferase, DNA-directed DNA polymerase, DNA replication, DNA damage, DNA repair, DNA-binding, Mutator protein, Metal-binding, Magnesium.

GO:0003677; Molecular function: DNA binding.
GO:0003887; Molecular function: DNA-directed DNA polymerase activity.
GO:0000287; Molecular function: magnesium ion binding.
GO:0006261; Biological process: DNA-dependent DNA replication.
GO:0006281; Biological process: DNA repair.
GO:0005737; Cellular component: cytoplasm.

From: DPO4_SULSF (P96022)
Key		From		To		Description		Condition		FTGroup
ACT_SITE		106		106		By similarity		E
METAL		7		7		Magnesium (By similarity)		D
METAL		105		105		Magnesium (By similarity)		D
SITE		12		12		Substrate discrimination (By similarity)

Size range:	318-479 amino acids
Related UniRules:	None
Template:	Q47155 (DPO4_ECOLI); P96022 (DPO4_SULSF); Q97W02 (DPO4_SULSO): [Recover all]
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	in AGRT5, BACHD, BACSU, MYCTU, RHILO, RHIME
Plasmid encoded:	in AGRT5, RHILO, RHIME