Annotation rule MF_01125
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General rule information [?]

Accession MF_01125
Dates 31-JAN-2004 (Created)
22-JUL-2013 (Last updated, Version 19)
Name Reverse_gyrase
Scope Bacteria
Archaea
Templates Q08582 (RGYR_SULAC); O29238 (RGYR_ARCFU): [Recover all]

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
RGYR
Protein name
RecName: Full=Reverse gyrase;
Includes:
RecName: Full=Helicase;
EC=3.6.4.12;
Includes:
RecName: Full=Topoisomerase;
EC=5.99.1.3;
Gene name
rgy

Comments [?]

Function Modifies the topological state of DNA by introducing positive supercoils in an ATP-dependent process. It cleaves transiently a single DNA strand and remains covalently bound to the 5' DNA end through a tyrosine residue. May be involved in rewinding the DNA strands in the regions of the chromosome that have opened up to allow transcription or replication (By similarity).
Catalytic activity ATP + H(2)O = ADP + phosphate.
ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit Monomer (By similarity).
Domain Both the DNA unwinding and positive supercoiling activities require the cooperation of both domains. The cooperative action between the helicase-like and the topoisomerase domains is specific. The helicase-like domain probably does not directly unwind DNA but acts more likely by driving ATP-dependent conformational changes within the whole enzyme, functioning more like a protein motor. The "latch" region of the N-terminal domain plays a regulatory role in the enzyme, repressing topoisomerase activity in the absence of ATP and therefore preventing the enzyme from acting as an ATP-independent relaxing enzyme; it also helps to coordinate nucleotide hydrolysis by the ATPase domain with the supercoiling activity of the topoisomerase domain (By similarity).
Miscellaneous This enzyme is the only unique feature of hyperthermophilic bacteria/archaea discovered so far. It appears to be essential for adaptation to life at high temperatures.
Similarity In the N-terminal section; belongs to the DEAD box helicase family. DDVD subfamily.
In the C-terminal section; belongs to the prokaryotic type I/III topoisomerase family.

Keywords [?]

case <FT:2> or <FT:4>
end case

Gene Ontology [?]

GO:0005524; Molecular function: ATP binding.
GO:0003677; Molecular function: DNA binding.
GO:0003918; Molecular function: DNA topoisomerase type II (ATP-hydrolyzing) activity.
GO:0004386; Molecular function: helicase activity.
case <FT:2> or <FT:4>
GO:0008270; Molecular function: zinc ion binding.
end case
GO:0006265; Biological process: DNA topological change.
GO:0006268; Biological process: DNA unwinding involved in DNA replication.

Cross-references [?]

Pfam PF00270; DEAD; 1;
PF01131; Topoisom_bac; 1;
PF01751; Toprim; 1;
PF00271; Helicase_C; 1;
PRINTS PR00417; PRTPISMRASEI; 1;
PROSITE PS51192; HELICASE_ATP_BIND_1; 1; trigger=PRU00541;
PS51194; HELICASE_CTER; 0-1; trigger=PRU00542;
PS50880; TOPRIM; 1; trigger=PRU00995;
TIGRFAMs TIGR01054; rgy; 1;

Features [?]

From: RGYR_PYRFU (P95479)
Key     From     To       Description   Tag   Condition   FTGroup
ZN_FING     9     30       C4-type 1 (By similarity)     C-x-x-C-x(10,18)-[CH]-x-x-C  
NP_BIND     106     113       ATP (By similarity)     x-P-x-G-x-G-K-[ST]  
ZN_FING     722     741       C4-type 2 (By similarity)     C-x-x-C-x(10,18)-C-x-x-C  
REGION     645     Cter       Topoisomerase I        
ACT_SITE     955     955       For DNA cleavage activity (By similarity)     Y  
BINDING     89     89       ATP (By similarity)     Q  

Additional information [?]

Size range 1054-1624 amino acids
Related rules None
Fusion None
Comments The reverse gyrases of M. jannaschii and P. horikoshii contain inteins which were excised in the alignment