HAMAP rule MF_01139
General rule information
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Accession | MF_01139 |
Dates | 1-SEP-2004 (Created)
1-MAR-2024 (Last updated, Version 37) |
Name | ISPT |
Scope(s) |
Bacteria Archaea |
Template(s) | P60472 (UPPS_ECOLI); O82827 (UPPS_MICLU); Q9HH76 (UPPS_SULAC); P9WFF7 (DPDS_MYCTU); A0R0S4 (DPDS_MYCS2); P9WFF5 (ZFPP_MYCTU); A0R2W4 (ZFPP_MYCS2); Q47SS3 (ZFPP_THEFY); Q47RM6 (DPDP_THEFY); [ Recover all ] |
Triggered by |
HAMAP; MF_01139 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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case <OC:Gammaproteobacteria> | |
Identifier | UPPS |
Protein name | RecName: Full=Ditrans,polycis-undecaprenyl-diphosphate synthase ((2E,6E)-farnesyl-diphosphate specific); EC=2.5.1.31; AltName: Full=Ditrans,polycis-undecaprenylcistransferase; AltName: Full=Undecaprenyl diphosphate synthase; Short=UDS; AltName: Full=Undecaprenyl pyrophosphate synthase; Short=UPP synthase; |
Gene name | Name=uppS; |
else case <OC:Archaea> | |
Identifier | UPPS |
Protein name | RecName: Full=Tritrans,polycis-undecaprenyl-diphosphate synthase (geranylgeranyl-diphosphate specific); EC=2.5.1.89; AltName: Full=Undecaprenyl diphosphate synthase; Short=UDS; AltName: Full=Undecaprenyl pyrophosphate synthase; Short=UPP synthase; |
Gene name | Name=uppS; |
else | |
Identifier | ISPT |
Protein name | RecName: Full=Isoprenyl transferase; EC=2.5.1.-; |
end case |
Comments
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case <OC:Gammaproteobacteria> | |
FUNCTION | Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with (2E,6E)-farnesyl diphosphate (E,E-FPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30Z,34E,38E)-undecaprenyl diphosphate (di- trans,octa-cis-UPP). UPP is the precursor of glycosyl carrier lipid in the biosynthesis of bacterial cell wall polysaccharide components such as peptidoglycan and lipopolysaccharide. |
CATALYTIC ACTIVITY | Reaction=(2E,6E)-farnesyl diphosphate + 8 isopentenyl diphosphate = di- trans,octa-cis-undecaprenyl diphosphate + 8 diphosphate; Xref=Rhea:RHEA:27551, ChEBI:CHEBI:33019, ChEBI:CHEBI:58405, ChEBI:CHEBI:128769, ChEBI:CHEBI:175763; EC=2.5.1.31; |
else case <OC:Archaea> | |
FUNCTION | Catalyzes the sequential condensation of isopentenyl diphosphate (IPP) with geranylgeranyl diphosphate (GGPP) to yield (2Z,6Z,10Z,14Z,18Z,22Z,26Z,30E,34E,38E)-undecaprenyl diphosphate (tritrans,heptacis-UPP). It is probably the precursor of glycosyl carrier lipids. |
CATALYTIC ACTIVITY | Reaction=geranylgeranyl diphosphate + 7 isopentenyl diphosphate = 7 diphosphate + tri-trans,hepta-cis-undecaprenyl diphosphate; Xref=Rhea:RHEA:27622, ChEBI:CHEBI:33019, ChEBI:CHEBI:57533, ChEBI:CHEBI:60388, ChEBI:CHEBI:128769; EC=2.5.1.89; |
else | |
FUNCTION | Catalyzes the condensation of isopentenyl diphosphate (IPP) with allylic pyrophosphates generating different type of terpenoids. |
end case | |
COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 2 magnesium ions per subunit.; |
SUBUNIT | Homodimer. |
SIMILARITY | Belongs to the UPP synthase family. |
Keywords
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Magnesium | |
Metal-binding | |
Transferase | |
case <OC:Gammaproteobacteria> | |
Cell shape | |
Cell wall biogenesis/degradation | |
Peptidoglycan synthesis | |
end case |
Gene Ontology
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case <OCellular component:Gammaproteobacteria> | |
GO:0008834; Molecular function:di-trans,poly-cis-undecaprenyl-diphosphate synthase activity | |
GO:0009252; Biological process:peptidoglycan biosynthetic process | |
end case | |
GO:0000287; Molecular function:magnesium ion binding | |
GO:0004659; Molecular function:prenyltransferase activity |
Cross-references
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Features
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From: UPPS_ECOLI (P60472) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 26 | 26 | D | |||||||||
ACT_SITE | 74 | 74 | /note="Proton acceptor" | N | ||||||||
BINDING | 27 | 30 | /ligand="substrate" | G-N-x-[RK] | ||||||||
BINDING | 71 | 73 | /ligand="substrate" | S-x-[ED] | ||||||||
BINDING | 200 | 202 | /ligand="substrate" | R-x-S | ||||||||
BINDING | 31 | 31 | /ligand="substrate" | [WF] | ||||||||
BINDING | 39 | 39 | /ligand="substrate" | [RK] | ||||||||
BINDING | 43 | 43 | /ligand="substrate" | H | ||||||||
BINDING | 75 | 75 | /ligand="substrate" | [WF] | ||||||||
BINDING | 77 | 77 | /ligand="substrate" | [RK] | ||||||||
BINDING | 194 | 194 | /ligand="substrate" | R | ||||||||
BINDING | 26 | 26 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
D | ||||||||
case <OC:Enterobacterales> | ||||||||||||
BINDING | 199 | 199 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
H | ||||||||
end case | ||||||||||||
BINDING | 213 | 213 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
E |
Additional information
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Size range | 219-339 amino acids |
Related rules |
None |
Fusion | Nter: <crtB> Cter: None |
Comments | Actinomycetota possess different types of isoprenyl transferases (decaprenyl diphosphate synthase; trans,polycis-decaprenyl diphosphate synthase; (2Z,6E)-farnesyl diphosphate synthase; trans,polycis-polyprenyl diphosphate synthase) which are all homologous to ISPT in primary sequence (and match the family MF_01139) but with a different substrate specificity (isoprenyl acceptor) and chain-length determination due to a few residues. |