HAMAP

Accession	MF_01152
Dates	12-APR-2005 (Created) 1-APR-2011 (Last updated, Version 15)

Data class

Protein

Predictors

HAMAP; MF_01152; [distribution of match scores in UniProtKB];[seed alignment for MF_01152]

Names

DnaJ

Identifier

DNAJ

Protein name

RecName: Full=Chaperone protein DnaJ;

Gene name

dnaJ

FUNCTION: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, DnaK-independent fashion. Unfolded proteins bind initially to DnaJ; upon interaction with the DnaJ-bound protein, DnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from DnaK; ATP binding to DnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding. Also involved, together with DnaK and GrpE, in the DNA replication of plasmids through activation of initiation proteins (By similarity).

COFACTOR: Binds 2 zinc ions per monomer (By similarity).

SUBUNIT: Homodimer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

DOMAIN: The J domain is necessary and sufficient to stimulate DnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, DnaK-independent chaperone activity of DnaJ. Zinc center 2 is essential for interaction with DnaK and for DnaJ activity (By similarity).

SIMILARITY: Belongs to the DnaJ family.

SIMILARITY: Contains 1 CR-type zinc finger.

SIMILARITY: Contains 1 J domain.

Pfam	PF00226; DnaJ; 1; PF01556; DnaJ_C; 1; PF00684; DnaJ_CXXCXGXG; 1;
PRINTS	PR00625; JDOMAIN; 1;
PROSITE	PS00636; DNAJ_1; 1; PS50076; DNAJ_2; 1; trigger=PRU00286; PS51188; ZF_CR; 1; trigger=PRU00546;

Cytoplasm, Chaperone, DNA replication, Stress response.

Metal-binding.

Repeat.

Zinc.

Zinc-finger.

GO:0051082; Molecular function: unfolded protein binding.

GO:0008270; Molecular function: zinc ion binding.

GO:0005737; Cellular component: cytoplasm.

From: DNAJ_ECOLI (P08622)
Key		From		To		Description		Condition		FTGroup
REPEAT		144		151		CXXCXGXG motif		C-x(2)-C-x-G-x-G		1
REPEAT		161		168		CXXCXGXG motif		C-x(2)-C-x-G-x-G		1
REPEAT		183		190		CXXCXGXG motif		C-x(2)-C-x-G-x-G		1
REPEAT		197		204		CXXCXGXG motif		C-x(2)-C-x-G-x-G		1
COMPBIAS		77		114		Gly-rich
METAL		144		144		Zinc 1 (By similarity)		C		2
METAL		147		147		Zinc 1 (By similarity)		C		2
METAL		161		161		Zinc 2 (By similarity)		C		3
METAL		164		164		Zinc 2 (By similarity)		C		3
METAL		183		183		Zinc 2 (By similarity)		C		3
METAL		186		186		Zinc 2 (By similarity)		C		3
METAL		197		197		Zinc 1 (By similarity)		C		2
METAL		200		200		Zinc 1 (By similarity)		C		2

Size range:	347-403 amino acids
Related UniRules:	None
Template:	P08622 (DNAJ_ECOLI)
Scope:	Bacteria Archaea; Euryarchaeota
Fusion:	Nter: None; Cter: None
Duplicate:	in AQUAE, AROAE, CORDI, COREF, CORGL, MYCBO, MYCLE, MYCPA, MYCTU, NOCFA, PROAC, STRAL, STRAW, STRCO, SYNY3
Plasmid encoded:	None