Annotation rule MF_01161
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General rule information [?]

Accession MF_01161
Dates 1-MAR-2005 (Created)
27-NOV-2013 (Last updated, Version 23)
Name tRNA_Ile_lys_synt
Scope Bacteria
Plastid

Propagated annotation [?]


Identifier, protein and gene names [?]

Identifier
TILS
case not <OG:Chloroplast>
Protein name
RecName: Full=tRNA(Ile)-lysidine synthase;
EC=6.3.4.19;
AltName: Full=tRNA(Ile)-2-lysyl-cytidine synthase;
AltName: Full=tRNA(Ile)-lysidine synthetase;
else case <OG:Chloroplast>
Protein name
RecName: Full=tRNA(Ile)-lysidine synthase, chloroplastic;
EC=6.3.4.19;
AltName: Full=tRNA(Ile)-2-lysyl-cytidine synthase;
AltName: Full=tRNA(Ile)-lysidine synthetase;
end case
Gene name
tilS

Comments [?]

Function Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionine to isoleucine (By similarity).
Catalytic activity (tRNA(Ile2))-cytidine(34) + L-lysine + ATP = (tRNA(Ile2))-lysidine(34) + AMP + diphosphate + H(2)O.
case <OC:Bacteria>
Subcellular location Cytoplasm (By similarity).
else case <OG:Chloroplast>
Subcellular location Plastid, chloroplast.
end case
case <FT:1>
Domain The N-terminal region contains the highly conserved SGGXDS motif, predicted to be a P-loop motif involved in ATP binding.
end case
Similarity Belongs to the tRNA(Ile)-lysidine synthase family.

Keywords [?]

case <FT:1>
end case
case <OC:Bacteria>
end case

Gene Ontology [?]

case <FT:1>
GO:0005524; Molecular function: ATP binding.
end case
GO:0016879; Molecular function: ligase activity, forming carbon-nitrogen bonds.
GO:0006400; Biological process: tRNA modification.
case <OC:Bacteria>
GO:0005737; Cellular component: cytoplasm.
else case <OG:Chloroplast>
GO:0009507; Cellular component: chloroplast.
end case

Cross-references [?]

Pfam PF01171; ATP_bind_3; 1;

Features [?]

From: TILS_ECOLI (P52097)
Key     From     To       Description   Tag   Condition   FTGroup
NP_BIND     20     25       ATP (Potential)     S-G-G-x-D-S  

Additional information [?]

Size range 278-648 amino acids
Related rules None
Template P52097 (TILS_ECOLI)
Fusion Nter: None; Cter: <hprT>, <CMP/dCMP deaminase zinc-binding domain>