HAMAP

Accession	MF_01209
Dates	4-JUL-2002 (Created) 31-MAR-2011 (Last updated, Version 40)

Data class

Protein

Predictors

HAMAP; MF_01209; [distribution of match scores in UniProtKB];[seed alignment for MF_01209]

Names

CPSase_S_chain

Identifier

CARA

Protein name

RecName: Full=Carbamoyl-phosphate synthase small chain; EC=6.3.5.5; AltName: Full=Carbamoyl-phosphate synthetase glutamine chain;

Gene name

carA

CATALYTIC ACTIVITY: 2 ATP + L-glutamine + HCO(3)(-) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.

PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

SUBUNIT: Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate (By similarity).

SUBCELLULAR LOCATION: Plastid, chloroplast.

SIMILARITY: Belongs to the CarA family.

Pfam	PF00117; GATase; 1; PF00988; CPSase_sm_chain; 1;
PRINTS	PR00099; CPSGATASE; 1; PR00096; GATASE; 1;
PROSITE	PS51273; GATASE_TYPE_1; 1; trigger=PRU00605;

Amino-acid biosynthesis, Arginine biosynthesis, ATP-binding, Glutamine amidotransferase, Ligase, Nucleotide-binding, Pyrimidine biosynthesis.

GO:0005524; Molecular function: ATP binding.
GO:0004088; Molecular function: carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity.
GO:0006526; Biological process: arginine biosynthetic process.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.

GO:0009507; Cellular component: chloroplast.

From: CARA_ECOLI (P0A6F1)
Key		From		To		Description		Condition		FTGroup
REGION		Nter		189		CPSase
ACT_SITE		269		269		Nucleophile (By similarity)		C
ACT_SITE		353		353		By similarity		H
ACT_SITE		355		355		By similarity		[ED]

Size range:	345-407 amino acids
Related UniRules:	None
Template:	P0A6F1 (CARA_ECOLI); P54324 (CARX_GEOSE); Q9L4N5 (CARA_LACLM); P77885 (CARA_LACPL); O08317 (CARX_LACPL): [Recover all]
Scope:	Bacteria Archaea Plastid
Fusion:	Nter: None; Cter: None
Duplicate:	in BACHD, BACSU, LACPL
Plasmid encoded:	None
Comments:	Bacillaceae, Geobacillus and lactobacillaceae seem to posess two members of this family: one specific for arginine biosynthesis (called CARX_ in ID, and CARA in GN) and the other specific for pyrimidine synthesis (called CARA_ in ID, and PYRAA in GN). Weird insert in THEMA; not shown in alignment.