HAMAP

Accession	MF_01210
Dates	19-JUL-2002 (Created) 22-DEC-2010 (Last updated, Version 39)

Data class

Protein

Predictors

HAMAP; MF_01210_B; [distribution of match scores in UniProtKB];[seed alignment for MF_01210_B]

Predictors

HAMAP; MF_01210_A; [distribution of match scores in UniProtKB];[seed alignment for MF_01210_A]

Names

CPSase_L_chain

Identifier

CARB

Protein name

RecName: Full=Carbamoyl-phosphate synthase large chain; EC=6.3.5.5; AltName: Full=Carbamoyl-phosphate synthetase ammonia chain;

Gene name

carB

CATALYTIC ACTIVITY: 2 ATP + L-glutamine + HCO(3)(-) + H(2)O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate.

PATHWAY: Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1.

PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3.

SUBUNIT: Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate (By similarity).

SIMILARITY: Belongs to the CarB family.

Pfam	PF00289; CPSase_L_chain; 1; PF02142; MGS; 1; PF02786; CPSase_L_D2; 1; PF02787; CPSase_L_D3; 1;
PRINTS	PR00098; CPSASE; 1;
PROSITE	PS00866; CPSASE_1; 1; PS00867; CPSASE_2; 1; PS50975; ATP_GRASP; 1-2; trigger=PRU00409;

Amino-acid biosynthesis, Arginine biosynthesis, Pyrimidine biosynthesis, Ligase, Repeat, ATP-binding, Nucleotide-binding.

GO:0005524; Molecular function: ATP binding.
GO:0004088; Molecular function: carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity.
GO:0006526; Biological process: arginine biosynthetic process.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.

From: CARB_ECOLI (P00968)
Key		From		To		Description		Condition		FTGroup
REGION		Nter		403		Carboxyphosphate synthetic domain
REGION		404		553		Oligomerization domain
REGION		554		936		Carbamoyl phosphate synthetic domain
REGION		937		Cter		Allosteric domain

From: CARB_SULTO (Q970U7)
Key		From		To		Description		Condition		FTGroup
REGION		Nter		399		Carboxyphosphate synthetic domain
REGION		400		548		Oligomerization domain
REGION		549		930		Carbamoyl phosphate synthetic domain
REGION		931		Cter		Allosteric domain

Size range:

1020-1167 amino acids

Size range:

1024-1076 amino acids

Related UniRules:	None
Template:	P00968 (CARB_ECOLI); O50302 (CARB_GEOSE); Q9ZB63 (CARY_GEOSE); O32771 (CARB_LACLM); P77886 (CARB_LACPL); Q9RLS9 (CARY_LACPL): [Recover all]
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	in BACHD, GEOSE, BACSU, LACPL
Plasmid encoded:	None
Comments:	Bacillaceae, Geobacillus and lactobacillaceae seem to posess two members of this family: one specific for arginine biosynthesis (called CARY_ in ID, and CARB in GN) and the other specific for pyrimidine synthesis (called CARB_ in ID, and PYRAB in GN). Sequence of carB is split into two genes in AQUAE, METJA and METKA. Weird insertion in NOSS1, THEMA and ZYMMO (sequences not shown in alignment).