HAMAP

Accession	MF_01211
Dates	17-JUN-2002 (Created) 19-JUL-2011 (Last updated, Version 36)

Data class

Protein

Predictors

HAMAP; MF_01211; [distribution of match scores in UniProtKB];[seed alignment for MF_01211]

Names

DHODB_Fe-S_bind

Identifier

PYRK

Protein name

RecName: Full=Dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit; AltName: Full=Dihydrdoorotate oxidase B, electron transfer subunit;

Protein name

RecName: Full=Probable dihydroorotate dehydrogenase B (NAD(+)), electron transfer subunit; AltName: Full=Dihydrdoorotate oxidase B, electron transfer subunit;

Gene name

pyrK

FUNCTION: Responsible for channeling the electrons from the oxidation of dihydroorotate from the FMN redox center in the PyrD type B subunit to the ultimate electron acceptor NAD(+) (By similarity).

COFACTOR: Binds 1 2Fe-2S cluster per subunit (By similarity).

COFACTOR: Binds 1 FAD per subunit (By similarity).

PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; orotate from (S)-dihydroorotate (NAD(+) route): step 1/1.

SUBUNIT: Heterotetramer of 2 PyrK and 2 PyrD type B subunits (By similarity).

SIMILARITY: Belongs to the PyrK family.

Pfam	PF10418; DHODB_Fe-S_bind; 1; PF00970; FAD_binding_6; 1; PF00175; NAD_binding_1; 1;
PIRSF	PIRSF006816; Cyc3_hyd_g; 1;
PRINTS	PR00409; PHDIOXRDTASE; 1;
PROSITE	PS00197; 2FE2S_FER_1; 0-1; PS51384; FAD_FR; 1; trigger=PRU00716;

Pyrimidine biosynthesis, Transport, Electron transport, Metal-binding, Iron, Iron-sulfur, 2Fe-2S, Flavoprotein, FAD.

GO:0009055; Molecular function: electron carrier activity.
GO:0006221; Biological process: pyrimidine nucleotide biosynthetic process.
GO:0055114; Biological process: oxidation-reduction process.

From: PYRK_LACLM (P56968)
Key		From		To		Description		Condition		FTGroup
NP_BIND		53		56		FAD (By similarity)		R-P-x-S
NP_BIND (Optional)		70		72		FAD (By similarity)		[LIV]-x-[RQ]
NP_BIND		79		80		FAD (By similarity)		G-T

Key		From		To		Description		Condition		FTGroup
METAL		226		226		Iron-sulfur (2Fe-2S) (By similarity)		C
METAL		231		231		Iron-sulfur (2Fe-2S) (By similarity)		C
METAL		234		234		Iron-sulfur (2Fe-2S) (By similarity)		C
METAL		249		249		Iron-sulfur (2Fe-2S) (By similarity)		C

Size range:	232-266 amino acids
Related UniRules:	None
Template:	P56968 (PYRK_LACLM); P25983 (PYRK_BACSU); P0DH76 (PYRK_ENTFA): [Recover all]
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	Divergent THEMA and THEP1, annotated as "atypical" since they are quite divergent from the family profile, but can be thought to be PyrK proteins as the gene is adjacent to pyrD.