HAMAP rule MF_01216

General rule information [?]

Accession	MF_01216
Dates	24-JUN-2002 (Created) 28-NOV-2023 (Last updated, Version 42)
Name	Azoreductase_type1
Scope(s)	Bacteria
Template(s)	P41407 (AZOR_ECOLI); Q9I5F3 (AZOR1_PSEAE); Q9I2E2 (AZOR2_PSEAE); Q9HZ17 (AZOR3_PSEAE); Q831B2 (AZOR_ENTFA); [ Recover all ]
Triggered by	HAMAP; MF_01216 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	AZOR
Protein name	RecName: Full=FMN dependent NADH:quinone oxidoreductase; EC=1.6.5.-; AltName: Full=Azo-dye reductase; AltName: Full=FMN-dependent NADH-azo compound oxidoreductase; AltName: Full=FMN-dependent NADH-azoreductase; EC=1.7.1.17;
Gene name	Name=azoR;

Comments [?]

FUNCTION	Quinone reductase that provides resistance to thiol-specific stress caused by electrophilic quinones.
FUNCTION	Also exhibits azoreductase activity. Catalyzes the reductive cleavage of the azo bond in aromatic azo compounds to the corresponding amines.
CATALYTIC ACTIVITY	Reaction=2 a quinone + H(+) + NADH = 2 a 1,4-benzosemiquinone + NAD(+); Xref=Rhea:RHEA:65952, ChEBI:CHEBI:15378, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124, ChEBI:CHEBI:134225;
CATALYTIC ACTIVITY	Reaction=anthranilate + N,N-dimethyl-1,4-phenylenediamine + 2 NAD(+) = 2-(4-dimethylaminophenyl)diazenylbenzoate + 2 H(+) + 2 NADH; Xref=Rhea:RHEA:55872, ChEBI:CHEBI:15378, ChEBI:CHEBI:15783, ChEBI:CHEBI:16567, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:71579; EC=1.7.1.17;
COFACTOR	Name=FMN; Xref=ChEBI:CHEBI:58210; Note=Binds 1 FMN per subunit.;
SUBUNIT	Homodimer.
SIMILARITY	Belongs to the azoreductase type 1 family.

Keywords [?]

Gene Ontology [?]

GO:0009055; Molecular function:electron transfer activity

GO:0010181; Molecular function:FMN binding

GO:0016652; Molecular function:oxidoreductase activity, acting on NAD(P)H, NAD(P) as acceptor

Cross-references [?]

Pfam	PF02525; Flavodoxin_2; 1;
PRINTS	PR00453; VWFADOMAIN; 1;

Features [?]

From: AZOR1_PSEAE (Q9I5F3)

Key

From

Description

Tag

Condition

FTGroup

BINDING

/ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"

S-x-S

BINDING

100

/ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"

M-[YW]-N-[FL]

BINDING

145

148

/ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"

[ST]-x-G-G

BINDING

/ligand="FMN"
/ligand_id="ChEBI:CHEBI:58210"

Additional information [?]

Size range	186-240 amino acids
Related rules	None
Fusion	Nter: None Cter: None
Comments	Divergent STRCO and first copy in CLOAB and CLOPE; sequences not included in alignment and not taken into account in size range. In some species there are many duplicates.