HAMAP rule MF_01225
General rule information
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Accession | MF_01225 |
Dates | 13-SEP-2002 (Created)
1-JUN-2023 (Last updated, Version 35) |
Name | MoaA |
Scope(s) |
Bacteria Archaea |
Template(s) | P69848 (MOAA_STAA8); Q44118 (MOAA_PAENI); P30745 (MOAA_ECOLI); Q9X5W3 (MOAA_RHOCA); [ Recover all ] |
Triggered by |
case c? <OC:Bacteria>
HAMAP; MF_01225_B (Get profile general information and statistics) end case
case c? <OC:Archaea>
HAMAP; MF_01225_A (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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Identifier | MOAA |
case <OC:Bacteria> | |
Protein name | RecName: Full=GTP 3',8-cyclase; EC=4.1.99.22; AltName: Full=Molybdenum cofactor biosynthesis protein A; |
end case | |
case <OC:Archaea> | |
Protein name | RecName: Full=Probable GTP 3',8-cyclase; EC=4.1.99.22; AltName: Full=Molybdenum cofactor biosynthesis protein A; |
end case | |
Gene name | Name=moaA; |
Comments
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FUNCTION | Catalyzes the cyclization of GTP to (8S)-3',8-cyclo-7,8- dihydroguanosine 5'-triphosphate. |
CATALYTIC ACTIVITY | Reaction=AH2 + GTP + S-adenosyl-L-methionine = (8S)-3',8-cyclo-7,8- dihydroguanosine 5'-triphosphate + 5'-deoxyadenosine + A + H(+) + L- methionine; Xref=Rhea:RHEA:49576, ChEBI:CHEBI:13193, ChEBI:CHEBI:15378, ChEBI:CHEBI:17319, ChEBI:CHEBI:17499, ChEBI:CHEBI:37565, ChEBI:CHEBI:57844, ChEBI:CHEBI:59789, ChEBI:CHEBI:131766; EC=4.1.99.22; |
COFACTOR | Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 2 [4Fe-4S] clusters. Binds 1 [4Fe-4S] cluster coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine and 1 [4Fe-4S] cluster coordinated with 3 cysteines and the GTP-derived substrate.; |
PATHWAY | Cofactor biosynthesis; molybdopterin biosynthesis. |
case <OC:Bacteria> | |
SUBUNIT | Monomer and homodimer. |
end case | |
SIMILARITY | Belongs to the radical SAM superfamily. MoaA family. |
Keywords
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4Fe-4S |
GTP-binding |
Iron |
Iron-sulfur |
Lyase |
Metal-binding |
Molybdenum cofactor biosynthesis |
Nucleotide-binding |
S-adenosyl-L-methionine |
Gene Ontology
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GO:0005525; Molecular function:GTP binding |
GO:0051539; Molecular function:4 iron, 4 sulfur cluster binding |
GO:0061798; Molecular function:GTP 3',8'-cyclase activity |
GO:1904047; Molecular function:S-adenosyl-L-methionine binding |
GO:0006777; Biological process:Mo-molybdopterin cofactor biosynthetic process |
Cross-references
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PROSITE | PS01305; MOAA_NIFB_PQQE; 1; |
Pfam | PF06463; Mob_synth_C; 1; |
Pfam | PF04055; Radical_SAM; 1; |
NCBIfam | TIGR02666; MoaA; 1; |
NCBIfam | TIGR02668; moaA_archaeal; 1; |
Features
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From: MOAA_STAA8 (P69848) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
case <OC:Bacteria> | ||||||||||||
BINDING | 266 | 268 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
[RK]-x-R | ||||||||
BINDING | 24 | 24 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
BINDING | 28 | 28 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
BINDING | 31 | 31 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
BINDING | 261 | 261 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-substrate" |
C | ||||||||
BINDING | 264 | 264 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-substrate" |
C | ||||||||
BINDING | 278 | 278 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-substrate" |
C | ||||||||
BINDING | 17 | 17 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
R | ||||||||
BINDING | 30 | 30 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
Y | ||||||||
BINDING | 71 | 71 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
R | ||||||||
BINDING | 75 | 75 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
G | ||||||||
BINDING | 102 | 102 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
[TS] | ||||||||
BINDING | 126 | 126 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
S | ||||||||
BINDING | 163 | 163 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
K | ||||||||
BINDING | 197 | 197 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
M | ||||||||
end case | ||||||||||||
From: MOAA_PYRAB (Q9V2G2) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
case <OC:Archaea> | ||||||||||||
BINDING | 255 | 257 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
R-x-R | ||||||||
BINDING | 21 | 21 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
BINDING | 25 | 25 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
BINDING | 28 | 28 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="1" /ligand_note="4Fe-4S-S-AdoMet" |
C | ||||||||
BINDING | 250 | 250 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-substrate" |
C | ||||||||
BINDING | 253 | 253 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-substrate" |
C | ||||||||
BINDING | 267 | 267 | /ligand="[4Fe-4S] cluster" /ligand_id="ChEBI:CHEBI:49883" /ligand_label="2" /ligand_note="4Fe-4S-substrate" |
C | ||||||||
BINDING | 14 | 14 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
R | ||||||||
BINDING | 27 | 27 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
Y | ||||||||
BINDING | 61 | 61 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
[KR] | ||||||||
BINDING | 65 | 65 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
G | ||||||||
BINDING | 90 | 90 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
T | ||||||||
BINDING | 114 | 114 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
S | ||||||||
BINDING | 150 | 150 | /ligand="GTP" /ligand_id="ChEBI:CHEBI:37565" |
K | ||||||||
BINDING | 189 | 189 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
M | ||||||||
end case |
Additional information
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Size range | 296-357 amino acids |
Related rules |
None |
Fusion | Nter: None Cter: None |