HAMAP annotation rule MF_01228
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Accession MF_01228
Dates 30-OCT-2002 (Created)
9-FEB-2012 (Last updated, Version 31)
Name Met_tRNA_synth_type2
Scope Bacteria
Triggered by



Identifier
SYM
Protein name
RecName: Full=Methionine--tRNA ligase;
EC=6.1.1.10;
AltName: Full=Methionyl-tRNA synthetase;
Short=MetRS;
Gene name
metG


FUNCTION Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation (By similarity).
CATALYTIC ACTIVITY ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).
case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)
COFACTOR Binds 1 zinc ion per subunit (By similarity).
end case
case <Feature:PS50886>
SUBUNIT Homodimer (By similarity).
end case
case not <Feature:PS50886> and <OC:Bacteria>
SUBUNIT Monomer (By similarity).
end case
SUBCELLULAR LOCATION Cytoplasm (By similarity).
case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)
SIMILARITY Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily.
end case
case not <FT:3>
SIMILARITY Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2B subfamily.
end case
case <Feature:PS50886>
SIMILARITY Contains 1 tRNA-binding domain.
end case


Cytoplasm
Aminoacyl-tRNA synthetase
Protein biosynthesis
Ligase
ATP-binding
Nucleotide-binding
case <Feature:PS50886>
RNA-binding
tRNA-binding
end case
case <FT:3> and <FT:4> and <FT:5> and (<FT:6> or <FT:7> or <FT:8>)
Metal-binding
Zinc
end case


GO:0005524; Molecular function: ATP binding.
GO:0004825; Molecular function: methionine-tRNA ligase activity.
GO:0006431; Biological process: methionyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.


Pfam PF00133; tRNA-synt_1; 1;
PRINTS PR01041; TRNASYNTHMET; 1;
TIGRFAMs TIGR00398; MetG; 1;
TIGR00399; MetG_C_term; 1;
PROSITE PS00178; AA_TRNA_LIGASE_I; 1;
PS50886; TRBD; 0-1; trigger=PRU00209;


From: SYM_THET8 (P23395)
Key     From     To       Description   Tag   Condition   FTGroup
MOTIF     12     22       "HIGH" region     [YDFAS]-Y-x(5)-H-[LIV]-G-[SHN]  
MOTIF     297     301       "KMSKS" region     K-[MI]-[SG]-K-[ST]  
METAL     127     127       Zinc (By similarity)     C  
METAL     130     130       Zinc (By similarity)     C  
METAL     144     144       Zinc (By similarity)     C  
METAL     147     147       Zinc (By similarity)     [HC]  
case not <FT:6>
From: SYM_SYNY3 (Q55729)
METAL     152     152       Zinc (By similarity)     [HC]  
end case
case not <FT:7>
From: SYM_MYCGE (P47267)
METAL     147     147       Zinc (By similarity)     [HC]  
end case
BINDING     300     300       ATP (By similarity)     K  


Size range: 497-683 amino acids
Related Rules: MF_00098 (SYM)
Templates: P23395 (SYM_THET8); O05593 (SYM_MYCTU); P23920 (SYM_GEOSE): [Recover all]
Fusion: None
Comments: Subfamily 2a links 1 zinc ion ion (shown in THET8) whereas subfamily 2b does not (shown in MYCTU). Possible wrong starts in AQUAE and SYNY3.


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