HAMAP

Accession	MF_01237
Dates	21-MAR-2003 (Created) 18-AUG-2010 (Last updated, Version 24)

Data class

Protein

Predictors

HAMAP; MF_01237; [distribution of match scores in UniProtKB];[seed alignment for MF_01237]

Names

N-acetylneuram_lyase

Identifier

NANA

Protein name

RecName: Full=N-acetylneuraminate lyase; EC=4.1.3.3; AltName: Full=N-acetylneuraminate pyruvate-lyase; AltName: Full=N-acetylneuraminic acid aldolase; AltName: Full=Sialate lyase; AltName: Full=Sialic acid aldolase; AltName: Full=Sialic acid lyase;

Gene name

nanA

FUNCTION: Catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetylmannosamine via a Schiff base intermediate (By similarity).

CATALYTIC ACTIVITY: N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.

PATHWAY: Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 1/5.

SUBUNIT: Homotetramer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: Belongs to the DHDPS family. NanA subfamily.

Pfam	PF00701; DHDPS; 1;
PRINTS	PR00146; DHPICSNTHASE; 1;
TIGRFAMs	TIGR00683; nanA; 1;
PROSITE	PS00665; DHDPS_1; 1; PS00666; DHDPS_2; 1;

Cytoplasm, Carbohydrate metabolism, Lyase, Schiff base.

GO:0008747; Molecular function: N-acetylneuraminate lyase activity.
GO:0005975; Biological process: carbohydrate metabolic process.
GO:0005737; Cellular component: cytoplasm.

From: NANA_ECOLI (P0A6L4)
Key		From		To		Description		Condition		FTGroup
REGION		47		48		Substrate binding (By similarity)		S-[TS]
ACT_SITE		165		165		Schiff-base intermediate with substrate (By similarity)		K
SITE		137		137		Involved in proton transfer during cleavage (By similarity)		Y

Size range:	288-305 amino acids
Related UniRules:	MF_00418 (DAPA); MF_00694 (KDGD)
Template:	P0A6L4 (NANA_ECOLI); P44539 (NANA_HAEIN); Q9S4K9 (NANA_CLOPE): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	in ECOL6
Plasmid encoded:	in RHIME