HAMAP rule MF_01246
General rule information
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| PURL | https://purl.expasy.org/hamap/rule/MF_01246 |
| Accession | MF_01246 |
| Dates | 19-JUN-2003 (Created)
3-SEP-2024 (Last updated, Version 29) |
| Name | COD |
| Scope(s) |
Bacteria |
| Template(s) | P37794 (CHBG_ECOLI); P59745 (YDJC_ENTFA); [ Recover all ] |
| Triggered by |
HAMAP; MF_01246 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| case <OC:Enterobacterales> | |
| Identifier | CHBG |
| Protein name | RecName: Full=Chitooligosaccharide deacetylase; Short=COD; EC=3.5.1.105; AltName: Full=Chitin disaccharide deacetylase; AltName: Full=Chitobiose deacetylase; AltName: Full=Chitobiose-6P deacetylase; AltName: Full=Chitotriose deacetylase; AltName: Full=Chitotriose-6P deacetylase; |
| Gene name | Name=chbG; |
| else | |
| Identifier | YDJC |
| Protein name | RecName: Full=Carbohydrate deacetylase; EC=3.5.1.-; |
| end case | |
Comments
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| case <OC:Enterobacterales> | |
| FUNCTION | Involved in the degradation of chitin. ChbG is essential for growth on the acetylated chitooligosaccharides chitobiose and chitotriose but is dispensable for growth on cellobiose and chitosan dimer, the deacetylated form of chitobiose. Deacetylation of chitobiose-6-P and chitotriose-6-P is necessary for both the activation of the chb promoter by the regulatory protein ChbR and the hydrolysis of phosphorylated beta-glucosides by the phospho-beta-glucosidase ChbF. Catalyzes the removal of only one acetyl group from chitobiose-6-P to yield monoacetylchitobiose-6-P, the inducer of ChbR and the substrate of ChbF. |
| CATALYTIC ACTIVITY | Reaction=N,N'-diacetylchitobiose + H2O = N-acetyl-beta-D-glucosaminyl- (1->4)-D-glucosamine + acetate; Xref=Rhea:RHEA:27469, ChEBI:CHEBI:15377, ChEBI:CHEBI:28681, ChEBI:CHEBI:30089, ChEBI:CHEBI:59910; EC=3.5.1.105; |
| CATALYTIC ACTIVITY | Reaction=diacetylchitobiose-6'-phosphate + H2O = N'- monoacetylchitobiose-6'-phosphate + acetate; Xref=Rhea:RHEA:35083, ChEBI:CHEBI:15377, ChEBI:CHEBI:30089, ChEBI:CHEBI:64883, ChEBI:CHEBI:71315; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| PATHWAY | Glycan degradation; chitin degradation. |
| SUBUNIT | Homodimer. |
| SUBCELLULAR LOCATION | Cytoplasm. |
| SIMILARITY | Belongs to the YdjC deacetylase family. ChbG subfamily. |
| else | |
| FUNCTION | Probably catalyzes the deacetylation of acetylated carbohydrates an important step in the degradation of oligosaccharides. |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; |
| SUBUNIT | Homodimer. |
| SIMILARITY | Belongs to the YdjC deacetylase family. |
| end case | |
Keywords
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| Carbohydrate metabolism | |
| case <OC:Enterobacterales> | |
| Chitin degradation | |
| Cytoplasm | |
| Polysaccharide degradation | |
| end case | |
| Hydrolase | |
| Magnesium | |
| Metal-binding | |
Gene Ontology
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| case <OCellular component:Enterobacterales> | |
| GO:0036311; Molecular function:chitin disaccharide deacetylase activity | |
| GO:0052777; Biological process:diacetylchitobiose catabolic process | |
| GO:0005737; Cellular component:cytoplasm | |
| GO:0000272; Biological process:polysaccharide catabolic process | |
| end case | |
| GO:0016811; Molecular function:hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amides | |
Cross-references
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| Pfam | PF04794; YdjC; 1; |
Features
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| From: CHBG_ECOLI (P37794) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 61 | 61 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
H | ||||||||
| BINDING | 125 | 125 | /ligand="Mg(2+)" /ligand_id="ChEBI:CHEBI:18420" |
H | ||||||||
Additional information
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| Size range | 230-262 amino acids |
| Related rules |
None |
| Fusion | Nter: None Cter: None |