HAMAP

Accession	MF_01253
Dates	9-DEC-2004 (Created) 19-AUG-2010 (Last updated, Version 14)

Data class

Protein

Predictors

HAMAP; MF_01253; [distribution of match scores in UniProtKB];[seed alignment for MF_01253]

Names

Endonuclease_8

Identifier

END8

Protein name

RecName: Full=Endonuclease 8; AltName: Full=DNA glycosylase/AP lyase Nei; EC=3.2.2.-; EC=4.2.99.18; AltName: Full=DNA-(apurinic or apyrimidinic site) lyase Nei; AltName: Full=Endonuclease VIII;

Gene name

nei

FUNCTION: Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized pyrimidines, such as thymine glycol, 5,6-dihydrouracil and 5,6-dihydrothymine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates (By similarity).

CATALYTIC ACTIVITY: Removes damaged bases from DNA, leaving an abasic site.

CATALYTIC ACTIVITY: The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate.

COFACTOR: Binds 1 zinc ion per subunit (By similarity).

SIMILARITY: Belongs to the FPG family.

SIMILARITY: Contains 1 FPG-type zinc finger.

Pfam	PF01149; Fapy_DNA_glyco; 1; PF06831; H2TH; 1; PF06827; zf-FPG_IleRS; 1;
PROSITE	PS51068; FPG_CAT; 1; PS01242; ZF_FPG_1; 1; PS51066; ZF_FPG_2; 1; trigger=PRU00391;

DNA damage, DNA repair, DNA-binding, Glycosidase, Hydrolase, Lyase, Multifunctional enzyme.

Metal-binding, Zinc, Zinc-finger.

GO:0003906; Molecular function: DNA-(apurinic or apyrimidinic site) lyase activity.
GO:0000703; Molecular function: oxidized pyrimidine base lesion DNA N-glycosylase activity.

GO:0008270; Molecular function: zinc ion binding.

GO:0006281; Biological process: DNA repair.

From: END8_ECOLI (P50465)
Key		From		To		Description		Condition		FTGroup
INIT_MET		1		1		Removed (By similarity)
ACT_SITE		2		2		Schiff-base intermediate with DNA (By similarity)		P
ACT_SITE		3		3		Proton donor (By similarity)		E
ACT_SITE		53		53		Proton donor; for beta-elimination activity (By similarity)		K
ACT_SITE		253		253		Proton donor; for delta-elimination activity (By similarity)		R
BINDING		70		70		DNA (By similarity)		Q
BINDING		125		125		DNA (By similarity)		R
BINDING		169		169		DNA (By similarity)		N

Size range:	262-263 amino acids
Related UniRules:	None
Template:	P50465 (END8_ECOLI)
Scope:	Bacteria; Enterobacteriaceae
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None