HAMAP

Accession	MF_01258
Dates	21-APR-2005 (Created) 24-SEP-2010 (Last updated, Version 10)

Data class

Protein

Predictors

HAMAP; MF_01258; [distribution of match scores in UniProtKB];[seed alignment for MF_01258]

Names

F420_ligase_CofE

Identifier

COFE

Protein name

RecName: Full=F420-0:gamma-glutamyl ligase; EC=6.3.2.-;

Gene name

cofE

FUNCTION: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives (By similarity).

CATALYTIC ACTIVITY: GTP + F420-0 + n L-glutamate = GDP + phosphate + F420-n.

COFACTOR: Divalent cation. The ion could be magnesium (By similarity).

COFACTOR: Monovalent cation. The ion could be potassium (By similarity).

PATHWAY: Cofactor biosynthesis; coenzyme F420 biosynthesis.

SUBUNIT: Homodimer (By similarity).

SIMILARITY: Belongs to the CofE family.

Pfam	PF01996; F420_ligase; 1;
TIGRFAMs	TIGR01916; F420_puta_dedox; 1;

GTP-binding, Ligase, Manganese, Nucleotide-binding, Potassium.

GO:0016881; Molecular function: acid-amino acid ligase activity.
GO:0009108; Biological process: coenzyme biosynthetic process.

Size range:	248-257 amino acids
Related UniRules:	MF_01259 (COFE (supersedes the current rule))
Template:	Q58178 (COFE_METJA)
Scope:	Archaea; Archaeoglobi Archaea; Halobacteria Archaea; Methanobacteria Archaea; Methanococci Archaea; Methanomicrobia Archaea; Methanopyri
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	CofE homologs are found in the genomes of all organisms currently known to produce coenzyme F420. These include not only the methanogenic archaea but also nonmethanogenic archaea such as Halobacteria and Archaeoglobus, as well as some eubacteria such as Streptomyces, Mycobacterium, Nocardia, and Thermobifida (see MF_01259).