To improve security and privacy, we are moving our web pages and services from HTTP to HTTPS.
To give users of web services time to transition to HTTPS, we will support separate HTTP and HTTPS services until the end of 2017.
From January 2018 most HTTP traffic will be automatically redirected to HTTPS. [more...]
View this page in https
Annotation rule MF_01260
Send feedback

General rule information [?]

Accession MF_01260
Dates 26-JUL-2005 (Created)
26-JUL-2017 (Last updated, Version 19)
Name Carboxylester
Scope Bacteria; Proteobacteria
Templates P13001 (BIOH_ECOLI); Q9K197 (BIOH_NEIMB); Q8GHL1 (BIOH_SERMA): [Recover all]

Propagated annotation [?]

Identifier, protein and gene names [?]

Protein name
RecName: Full=Pimeloyl-[acyl-carrier protein] methyl ester esterase;
AltName: Full=Biotin synthesis protein BioH;
AltName: Full=Carboxylesterase BioH;
Gene name

Comments [?]

Function The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters.
Catalytic activity Pimeloyl-[acyl-carrier protein] methyl ester + H(2)O = pimeloyl-[acyl-carrier protein] + methanol.
Pathway Cofactor biosynthesis; biotin biosynthesis.
Subunit Monomer.
Subcellular location Cytoplasm.
Similarity Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.

Keywords [?]

Gene Ontology [?]

GO:0052689; Molecular function: carboxylic ester hydrolase activity.
GO:0009102; Biological process: biotin biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

Cross-references [?]

Pfam PF00561; Abhydrolase_1; 1;
PF00975; Thioesterase; 1;
TIGRFAMs TIGR01738; BioH; 1;

Features [?]

From: BIOH_ECOLI (P13001)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     82     83       Substrate binding     S-[LM]  
REGION     143     147       Substrate binding     F-x-x-x-[QD]  
ACT_SITE     82     82       Nucleophile     S  
ACT_SITE     207     207             D  
ACT_SITE     235     235             H  
BINDING     22     22       Substrate; via amide nitrogen and carbonyl oxygen     W  
BINDING     235     235       Substrate     H  

Additional information [?]

Size range 239-312 amino acids
Related rules None
Fusion Nter: None; Cter: MF_00835 (BioC)
Comments Longer C-terminal in NEIMA; sequence not shown in alignment and not taken into account in size range