HAMAP annotation rule MF_01260
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Accession MF_01260
Dates 26-JUL-2005 (Created)
11-JUL-2011 (Last updated, Version 15)
Name Carboxylester
Scope Bacteria; Proteobacteria
Triggered by



Identifier
BIOH
Protein name
RecName: Full=Pimelyl-[acyl-carrier protein] methyl ester esterase;
EC=3.1.1.85;
AltName: Full=Biotin synthesis protein BioH;
AltName: Full=Carboxylesterase BioH;
Gene name
bioH


FUNCTION The physiological role of BioH is to remove the methyl group introduced by BioC when the pimeloyl moiety is complete. It allows to synthesize pimeloyl-ACP via the fatty acid synthetic pathway through the hydrolysis of the ester bonds of pimeloyl-ACP esters (By similarity).
CATALYTIC ACTIVITY Pimelyl-[acyl-carrier protein] methyl ester + H(2)O = pimelyl-[acyl-carrier protein] + methanol.
PATHWAY Cofactor biosynthesis; biotin biosynthesis.
SUBUNIT Monomer (By similarity).
SUBCELLULAR LOCATION Cytoplasm (By similarity).
SIMILARITY Belongs to the AB hydrolase superfamily. Carboxylesterase BioH family.


Cytoplasm
Biotin biosynthesis
Hydrolase
Serine esterase


GO:0004091; Molecular function: carboxylesterase activity.
GO:0009102; Biological process: biotin biosynthetic process.
GO:0005737; Cellular component: cytoplasm.


Pfam PF00561; Abhydrolase_1; 1;
PF00975; Thioesterase; 1;
PRINTS PR00111; ABHYDROLASE; 1;
PR00412; EPOXHYDRLASE; 1;
TIGRFAMs TIGR01738; BioH; 1;


From: BIOH_ECOLI (P13001)
Key     From     To       Description   Tag   Condition   FTGroup
REGION     82     83       Substrate binding (By similarity)     S-[LM]  
REGION     143     147       Substrate binding (By similarity)     F-X-X-X-[QD]  
ACT_SITE     82     82       Nucleophile (By similarity)     S  
ACT_SITE     207     207       By similarity     D  
ACT_SITE     235     235       By similarity     H  
BINDING     22     22       Substrate; via amide nitrogen and carbonyl oxygen (By similarity)     W  
BINDING     235     235       Substrate (By similarity)     H  


Size range: 239-312 amino acids
Related Rules: None
Templates: P13001 (BIOH_ECOLI); Q9K197 (BIOH_NEIMB); Q8GHL1 (BIOH_SERMA): [Recover all]
Fusion: Nter: None; Cter: MF_00835 (BioC)
Comments: Longer C-terminal in NEIMA; sequence not shown in alignment and not taken into account in size range


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