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HAMAP rule MF_01269

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General rule information [?]

Accession MF_01269
Dates 18-MAY-2006 (Created)
19-NOV-2022 (Last updated, Version 21)
Name Shikimate_kinase_2
Scope(s) Bacteria
Enterobacterales
Template(s) P10880 (AROL_DICCH); P0A6E1 (AROL_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01269 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier AROL
Protein name RecName: Full=Shikimate kinase 2;
                 Short=SK 2;
                 EC=2.7.1.71;
Gene name Name=aroL;

Comments [?]

FUNCTIONCatalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate.
CATALYTIC ACTIVITY Reaction=ATP + shikimate = 3-phosphoshikimate + ADP + H(+); Xref=Rhea:RHEA:13121, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:36208, ChEBI:CHEBI:145989, ChEBI:CHEBI:456216; EC=2.7.1.71;
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
PATHWAYMetabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.
SUBUNITMonomer.
SUBCELLULAR LOCATIONCytoplasm.
DOMAINThe LID domain closes over the active site upon ATP binding.
SIMILARITYBelongs to the shikimate kinase family. AroL subfamily.

Keywords [?]


Gene Ontology [?]

GO:0000287; Molecular function:magnesium ion binding
GO:0005524; Molecular function:ATP binding
GO:0004765; Molecular function:shikimate kinase activity
GO:0009073; Biological process:aromatic amino acid family biosynthetic process
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

PROSITE PS01128; SHIKIMATE_KINASE; 1;
Pfam PF01202; SKI; 1;
PRINTS PR01100; SHIKIMTKNASE; 1;

Features [?]

From: AROL_DICCH (P10880)
Key From To Description Tag Condition FTGroup
BINDING 12 17 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
G-[CA]-G-K-T-T
REGION 112 126 /note="LID domain"
BINDING 16 16 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
T
BINDING 32 32 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
D
BINDING 34 34 /ligand="substrate" D
BINDING 58 58 /ligand="substrate" R
BINDING 79 79 /ligand="substrate" G
BINDING 120 120 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
R
BINDING 139 139 /ligand="substrate" R
BINDING 155 155 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
Q

Additional information [?]

Size range 173-181 amino acids
Related rules MF_00109
Fusion Nter: None Cter: None
Comments Mg(2+) is six-coordinated in shikimate kinase 1 (aroK, MF_00109) with direct interaction with two protein side-chains, whereas it is four-coordinated in shikimate kinase 2 (aroL, MF_01269) with direct interaction with only one protein side-chain.



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