HAMAP

Accession	MF_01269
Dates	18-MAY-2006 (Created) 15-MAR-2010 (Last updated, Version 12)

Data class

Protein

Predictors

HAMAP; MF_01269; [distribution of match scores in UniProtKB];[seed alignment for MF_01269]

Names

Shikimate_kinase_2

Identifier

AROL

Protein name

RecName: Full=Shikimate kinase 2; Short=SK 2; EC=2.7.1.71;

Gene name

aroL

FUNCTION: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate (By similarity).

CATALYTIC ACTIVITY: ATP + shikimate = ADP + shikimate 3-phosphate.

COFACTOR: Binds 1 magnesium ion per subunit (By similarity).

PATHWAY: Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7.

SUBUNIT: Monomer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

DOMAIN: The LID domain closes over the active site upon ATP binding (By similarity).

SIMILARITY: Belongs to the shikimate kinase family. AroL subfamily.

PROSITE	PS01128; SHIKIMATE_KINASE; 1;
Pfam	PF01202; SKI; 1;
PRINTS	PR01100; SHIKIMTKNASE; 1;

Cytoplasm, Amino-acid biosynthesis, Aromatic amino acid biosynthesis, ATP-binding, Kinase, Magnesium, Metal-binding, Nucleotide-binding, Transferase.

GO:0000287; Molecular function: magnesium ion binding.
GO:0005524; Molecular function: ATP binding.
GO:0004765; Molecular function: shikimate kinase activity.
GO:0009073; Biological process: aromatic amino acid family biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

From: AROL_ERWCH (P10880)
Key		From		To		Description		Condition		FTGroup
NP_BIND		12		17		ATP (By similarity)		G-[CA]-G-K-T-T
REGION		112		126		LID domain
METAL		16		16		Magnesium (By similarity)		T
METAL		32		32		Magnesium (By similarity)		D
BINDING		34		34		Substrate (By similarity)		D
BINDING		58		58		Substrate (By similarity)		R
BINDING		79		79		Substrate; via amide nitrogen (By similarity)		G
BINDING		120		120		ATP (By similarity)		R
BINDING		139		139		Substrate (By similarity)		R
BINDING (Optional)		155		155		ATP (By similarity)		Q

Size range:	173-181 amino acids
Related UniRules:	MF_00109 (AROK)
Template:	P10880 (AROL_ERWCH); P0A6E1 (AROL_ECOLI): [Recover all]
Scope:	Bacteria; Enterobacteriaceae
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	Mg(2+) is six-coordinated in shikimate kinase 1 (aroK, MF_00109) with direct interaction with two protein side-chains, whereas it is four-coordinated in shikimate kinase 2 (aroL, MF_01269) with direct interaction with only one protein side-chain.