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HAMAP rule MF_01272

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General rule information [?]

Accession MF_01272
Dates 6-NOV-2006 (Created)
13-JAN-2023 (Last updated, Version 23)
Name Heme_degrading_monooxygenase
Scope(s) Bacteria
Bacillota
Template(s) Q8NX62 (HDOX1_STAAW); Q2FZE2 (HDOX1_STAA8); Q2G1J2 (HDOX2_STAA8); Q99X56 (HDOX2_STAAM); Q81L50 (HDOX_BACAN); [ Recover all ]
Triggered by HAMAP; MF_01272 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier HDOX
case <OC:Staphylococcus>
Protein name RecName: Full=Heme oxygenase (staphylobilin-producing);
                 EC=1.14.99.48;
AltName: Full=Heme-degrading monooxygenase;
AltName: Full=Iron-regulated surface determinant;
AltName: Full=Iron-responsive surface determinant;
else
Protein name RecName: Full=Heme-degrading monooxygenase;
                 EC=1.14.14.18;
AltName: Full=Heme oxygenase;
AltName: Full=Iron-regulated surface determinant;
AltName: Full=Iron-responsive surface determinant;
Gene name Name=isdG;
end case

Comments [?]

case <OC:Staphylococcus>
FUNCTIONAllows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring to the oxo-bilirubin chromophore staphylobilin (a mixture of the linear tetrapyrroles 5-oxo-delta-bilirubin and 15-oxo- beta-bilirubin) in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron.
CATALYTIC ACTIVITY Reaction=5 AH2 + 2 H(+) + heme b + 4 O2 = 5 A + delta-staphylobilin + Fe(2+) + formaldehyde + 4 H2O; Xref=Rhea:RHEA:37039, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16842, ChEBI:CHEBI:17499, ChEBI:CHEBI:29033, ChEBI:CHEBI:60344, ChEBI:CHEBI:74361; EC=1.14.99.48;
CATALYTIC ACTIVITY Reaction=5 AH2 + 2 H(+) + heme b + 4 O2 = 5 A + beta-staphylobilin + Fe(2+) + formaldehyde + 4 H2O; Xref=Rhea:RHEA:37363, ChEBI:CHEBI:13193, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16842, ChEBI:CHEBI:17499, ChEBI:CHEBI:29033, ChEBI:CHEBI:60344, ChEBI:CHEBI:74362; EC=1.14.99.48;
else
FUNCTIONAllows bacterial pathogens to use the host heme as an iron source. Catalyzes the oxidative degradation of the heme macrocyclic porphyrin ring to the biliverdin in the presence of a suitable electron donor such as ascorbate or NADPH--cytochrome P450 reductase, with subsequent release of free iron.
CATALYTIC ACTIVITY Reaction=heme b + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = biliverdin IXalpha + CO + Fe(2+) + H(+) + 3 H2O + 3 oxidized [NADPH-- hemoprotein reductase]; Xref=Rhea:RHEA:21764, Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17245, ChEBI:CHEBI:29033, ChEBI:CHEBI:57618, ChEBI:CHEBI:57991, ChEBI:CHEBI:58210, ChEBI:CHEBI:60344; EC=1.14.14.18;
end case
SUBUNITHomodimer.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the antibiotic biosynthesis monooxygenase family. Heme-degrading monooxygenase IsdG subfamily.

Keywords [?]


Gene Ontology [?]

GO:0005506; Molecular function:iron ion binding
GO:0004392; Molecular function:heme oxygenase (decyclizing) activity
GO:0016705; Molecular function:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
GO:0020037; Molecular function:heme binding
GO:0005737; Cellular component:cytoplasm
GO:0042167; Biological process:heme catabolic process

Cross-references [?]

PROSITE PS51725; ABM; 1;
Pfam PF03992; ABM; 1;

Features [?]

From: HDOX2_STAAN (Q7A827)
Key From To Description Tag Condition FTGroup
BINDING 21 28 /ligand="heme"
/ligand_id="ChEBI:CHEBI:30413"
R-F-Y-x-R-x-G-I
BINDING 6 6 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"
N
BINDING 76 76 /ligand="heme"
/ligand_id="ChEBI:CHEBI:30413"
/ligand_part="Fe"
/ligand_part_id="ChEBI:CHEBI:18248"
/note="axial binding residue"
H
SITE 66 66 /note="Transition state stabilizer"

Additional information [?]

Size range 100-121 amino acids
Related rules None
Fusion Nter: None Cter: None
Comments Most Staphylococcus aureus strains have two family members; the second one is called isdI.



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