HAMAP

Accession	MF_01292
Dates	3-OCT-2008 (Created) 18-NOV-2010 (Last updated, Version 5)

Data class

Protein

Predictors

HAMAP; MF_01292; [distribution of match scores in UniProtKB];[seed alignment for MF_01292]

Names

HKHD_aldolase

Identifier

HPCH

Protein name

RecName: Full=4-hydroxy-2-oxo-heptane-1,7-dioate aldolase; EC=4.1.2.n4; AltName: Full=2,4-dihydroxyhept-2-ene-1,7-dioic acid aldolase; Short=HHED aldolase; AltName: Full=4-hydroxy-2-ketoheptane-1,7-dioate aldolase; Short=HKHD aldolase;

Gene name

hpcH, hpaI

FUNCTION: Catalyzes the reversible retro-aldol cleavage of 4-hydroxy-2-ketoheptane-1,7-dioate (HKHD) to pyruvate and succinic semialdehyde (By similarity).

CATALYTIC ACTIVITY: 4-hydroxy-2-ketoheptane-1,7-dioate = pyruvate + succinate semialdehyde.

COFACTOR: Binds 1 divalent metal cation per subunit (By similarity).

PATHWAY: Aromatic compound metabolism; 4-hydroxyphenylacetate degradation; pyruvate and succinate semialdehyde from 4-hydroxyphenylacetate: step 7/7.

SUBUNIT: Homohexamer; trimer of dimers (By similarity).

SIMILARITY: Belongs to the HpcH/HpaI aldolase family.

Pfam	PF03328; HpcH_HpaI; 1;
TIGRFAMs	TIGR02311; HpaI; 1;

Aromatic hydrocarbons catabolism, Lyase, Metal-binding.

GO:0016832; Molecular function: aldehyde-lyase activity.
GO:0046872; Molecular function: metal ion binding.
GO:0019439; Biological process: aromatic compound catabolic process.

From: HPCH_ECOLX (Q47098)
Key		From		To		Description		Condition		FTGroup
ACT_SITE		45		45		Proton acceptor (By similarity)		H
METAL		149		149		Divalent metal cation (By similarity)		E
METAL		175		175		Divalent metal cation (By similarity)		D
BINDING		147		147		Substrate (By similarity)		Q
BINDING		174		174		Substrate; via amide nitrogen (By similarity)		A
BINDING		175		175		Substrate (By similarity)		D
SITE		70		70		Transition state stabilizer (By similarity)		R
SITE		84		84		Increases basicity of active site His (By similarity)		D

Size range:	262-267 amino acids
Related UniRules:	None
Template:	Q47098 (HPCH_ECOLX)
Scope:	Bacteria; Enterobacteriales
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None