HAMAP rule MF_01297

General rule information [?]

Accession	MF_01297
Dates	25-NOV-2008 (Created) 13-JAN-2023 (Last updated, Version 15)
Name	nitrobindin
Scope(s)	Bacteria Actinomycetota
Template(s)	P9WFG9 (NB2_MYCTU); P9WFG7 (NB1_MYCTU); [ Recover all ]
Triggered by	HAMAP; MF_01297 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <FTTag:heme_iron_binding>
Identifier	NB
Protein name	RecName: Full=Peroxynitrite isomerase; EC=5.99.-.-; AltName: Full=Ferric nitrobindin; Short=Nb(III);
else
Protein name	RecName: Full=Ferric nitrobindin-like protein;
end case

Comments [?]

case <FTTag:heme_iron_binding>
FUNCTION	Heme-binding protein able to scavenge peroxynitrite and to protect free L-tyrosine against peroxynitrite-mediated nitration, by acting as a peroxynitrite isomerase that converts peroxynitrite to nitrate. Therefore, this protein likely plays a role in peroxynitrite sensing and in the detoxification of reactive nitrogen and oxygen species (RNS and ROS, respectively). Is able to bind nitric oxide (NO) in vitro, but may act as a sensor of peroxynitrite levels in vivo.
CATALYTIC ACTIVITY	Reaction=peroxynitrite = nitrate; Xref=Rhea:RHEA:63116, ChEBI:CHEBI:17632, ChEBI:CHEBI:25941; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:63117;
COFACTOR	Name=heme b; Xref=ChEBI:CHEBI:60344; Note=Binds 1 heme b group per subunit, that coordinates a highly solvent-exposed Fe(III) atom.
PATHWAY	Nitrogen metabolism.
DOMAIN	Forms a 10-stranded antiparallel beta-barrel structure able to accommodate a hydrophobic ligand in its interior. In fact, this fold hosts the heme group, which is located in a wide surface cleft.
else
CAUTION	Lacks the conserved His residue that binds heme iron in the nitrobindin family.
end case
SIMILARITY	Belongs to the nitrobindin family.

Keywords [?]

Gene Ontology [?]

case <FTTag:heme_iron_binding>
GO:0020037; Molecular function:heme binding
GO:0062213; Molecular function:peroxynitrite isomerase activity
end case

Cross-references [?]

Pfam	PF08768; DUF1794; 1;

Features [?]

From: NB1_MYCTU (P9WFG7)

Key

From

Description

Tag

Condition

FTGroup

MOTIF

/note="GXWXGXG"

G-x-W-x-G-x-G

BINDING

155

/ligand="heme b"
/ligand_id="ChEBI:CHEBI:60344"
/ligand_part="Fe"
/ligand_part_id="ChEBI:CHEBI:18248"
/note="axial binding residue"

heme_iron_binding

BINDING

/ligand="heme b"
/ligand_id="ChEBI:CHEBI:60344"

BINDING

123

/ligand="heme b"
/ligand_id="ChEBI:CHEBI:60344"

Additional information [?]