HAMAP

Accession	MF_01312
Dates	20-MAR-2003 (Created) 2-DEC-2010 (Last updated, Version 29)

Data class

Protein

Predictors

HAMAP; MF_01312; [distribution of match scores in UniProtKB];[seed alignment for MF_01312]

Names

NorV

Identifier

NORV

Protein name

RecName: Full=Anaerobic nitric oxide reductase flavorubredoxin; Short=FlRd; Short=FlavoRb;

Gene name

norV, flrD

FUNCTION: Anaerobic nitric oxide reductase; uses NADH to detoxify nitric oxide (NO), protecting several 4Fe-4S NO-sensitive enzymes. Has at least 2 reductase partners, only one of which (NorW, flavorubredoxin reductase) has been identified. NO probably binds to the di-iron center; electrons enter from the NorW at rubredoxin and are transferred sequentially to the FMN center and the di-iron center. Also able to function as an aerobic oxygen reductase (By similarity).

COFACTOR: Binds 3 iron ions per monomer (By similarity).

COFACTOR: Binds 1 FMN per monomer (By similarity).

PATHWAY: Nitrogen metabolism; nitric oxide reduction.

SUBUNIT: Homotetramer (By similarity).

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: In the N-terminal section; belongs to the zinc metallo-hydrolase group 3 family.

SIMILARITY: Contains 1 flavodoxin-like domain.

SIMILARITY: Contains 1 rubredoxin-like domain.

Pfam	PF00258; Flavodoxin_1; 1; PF00753; Lactamase_B; 1; PF00301; Rubredoxin; 1; PF00070; Pyr_redox; 1;
PIRSF	PIRSF005243; ROO; 1;
PRINTS	PR00163; RUBREDOXIN; 1;
PROSITE	PS50902; FLAVODOXIN_LIKE; 1; trigger=PRU00088; PS50903; RUBREDOXIN_LIKE; 1;

Cytoplasm, Transport, Electron transport, Oxidoreductase.

Flavoprotein, FMN.

Metal-binding, Iron.

GO:0009055; Molecular function: electron carrier activity.
GO:0016491; Molecular function: oxidoreductase activity.
GO:0055114; Biological process: oxidation-reduction process.
GO:0005737; Cellular component: cytoplasm.

From: NORV_ECOLI (Q46877)
Key		From		To		Description		Condition		FTGroup
DOMAIN		423		474		Rubredoxin-like
REGION		30		210		Zinc metallo-hydrolase
METAL		79		79		Iron 1 (By similarity)		H		1
METAL		81		81		Iron 1 (By similarity)		E		1
METAL		83		83		Iron 2 (By similarity)		D		1
METAL		147		147		Iron 1 (By similarity)		H		1
METAL		166		166		Iron 1 (By similarity)		D		1
METAL		166		166		Iron 2 (By similarity)		D		1
METAL		227		227		Iron 2 (By similarity)		H		1
METAL		428		428		Iron 3 (By similarity)		C		2
METAL		431		431		Iron 3 (By similarity)		C		2
METAL		461		461		Iron 3 (By similarity)		C		2
METAL		464		464		Iron 3 (By similarity)		C		2

Size range:	479-521 amino acids
Related UniRules:	None
Template:	Q46877 (NORV_ECOLI)
Scope:	Bacteria; Aeromonas Bacteria; Aliivibrio Bacteria; Citrobacter Bacteria; Escherichia Bacteria; Enterobacter Bacteria; Klebsiella Bacteria; Pectobacterium Bacteria; Salmonella Bacteria; Serratia Bacteria; Shigella Bacteria; Vibrio
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	ECO57 lacks the flavodoxin-like domain and is not used in alignment. Restricted to lineages where there is norR upstream and norW downstream.