HAMAP rule MF_01324

General rule information [?]

Accession	MF_01324
Dates	6-JAN-2004 (Created) 1-JUN-2023 (Last updated, Version 38)
Name	RNApol_bact_RpoC2
Scope(s)	Bacteria Cyanobacteriota Plastid
Template(s)	P22705 (RPOC2_NOSS1); [ Recover all ]
Triggered by	case c? <OC:Cyanobacteriota> or <OG:Chloroplast> HAMAP; MF_01324 (Get profile general information and statistics) end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	RPOC2
case <OC:Cyanobacteriota>
Protein name	RecName: Full=DNA-directed RNA polymerase subunit beta'; Short=RNAP subunit beta'; EC=2.7.7.6; AltName: Full=RNA polymerase subunit beta'; AltName: Full=Transcriptase subunit beta';
else case <OG:Chloroplast>
Protein name	RecName: Full=DNA-directed RNA polymerase subunit beta''; EC=2.7.7.6; AltName: Full=PEP; AltName: Full=Plastid-encoded RNA polymerase subunit beta''; Short=RNA polymerase subunit beta'';
end case
Gene name	Name=rpoC2;

Comments [?]

FUNCTION	DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates.
CATALYTIC ACTIVITY	Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate + RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA- COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395; EC=2.7.7.6;
case <FTGroup:1>
COFACTOR	Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Note=Binds 1 Zn(2+) ion per subunit.;
end case
case <OC:Cyanobacteriota>
SUBUNIT	In cyanobacteria the RNAP catalytic core is composed of 2 alpha, 1 beta, 1 beta', 1 gamma and 1 omega subunit. When a sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
else case <OG:Chloroplast>
SUBUNIT	In plastids the minimal PEP RNA polymerase catalytic core is composed of four subunits: alpha, beta, beta', and beta''. When a (nuclear-encoded) sigma factor is associated with the core the holoenzyme is formed, which can initiate transcription.
SUBCELLULAR LOCATION	Plastid, chloroplast.
end case
SIMILARITY	Belongs to the RNA polymerase beta' chain family. RpoC2 subfamily.

Keywords [?]

Gene Ontology [?]

GO:0003677; Molecular function:DNA binding
GO:0003899; Molecular function:DNA-directed 5'-3' RNA polymerase activity
GO:0006351; Biological process:DNA-templated transcription
case <FTGroup:1>
GO:0008270; Molecular function:zinc ion binding
end case
case <OG:Chloroplast>
GO:0009507; Cellular component:chloroplast
end case

Cross-references [?]

Features [?]

From: RPOC2_NOSS1 (P22705)

Key

From

Description

Tag

Condition

FTGroup

BINDING

219

/ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"

BINDING

293

/ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"

BINDING

300

/ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"

BINDING

303

/ligand="Zn(2+)"
/ligand_id="ChEBI:CHEBI:29105"

Additional information [?]

Size range	1011-1595 amino acids
Related rules	MF_01322
Fusion	Nter: MF_01323 (rpoC1) Cter: None
Comments	In cyanobacteria and plastids, there are two ORFs (rpoC1, MF_01323 and this one, rpoC2, MF_01324) that together are equivalent to the beta' subunit found in all other bacteria (rpoC, MF_01322). However,in the red algae CYAM1 the rpoC1 and rpoC2 genes are fused into one ORF. This seems to be the result of evolution acting to reduce protein size rather than horizontal transfer from a bacteria. Some algal proteins with inserts are not included in the alignment. ASTLO and EUGGR have large deletions and are not included in the alignments.