HAMAP

Accession	MF_01327
Dates	19-FEB-2001 (Created) 13-DEC-2011 (Last updated, Version 22)

Data class

Protein

Predictors

HAMAP; MF_01327; [distribution of match scores in UniProtKB];[seed alignment for MF_01327]

Names

TMP_synthase_cyanobact

Identifier

THIE

Protein name

RecName: Full=Thiamine-phosphate synthase; Short=TP synthase; Short=TPS; EC=2.5.1.3; AltName: Full=Thiamine-phosphate pyrophosphorylase; Short=TMP pyrophosphorylase; Short=TMP-PPase;

Gene name

thiE

FUNCTION: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) (By similarity).

CATALYTIC ACTIVITY: 2-methyl-4-amino-5-hydroxymethylpyrimidine diphosphate + 4-methyl-5-(2-phosphono-oxyethyl)thiazole = diphosphate + thiamine phosphate.

COFACTOR: Binds 1 magnesium ion per subunit (By similarity).

PATHWAY: Cofactor biosynthesis; thiamine diphosphate biosynthesis; thiamine phosphate from 4-amino-2-methyl-5-diphosphomethylpyrimidine and 4-methyl-5-(2-phosphoethyl)-thiazole: step 1/1.

SIMILARITY: Belongs to the thiamine-phosphate synthase family.

Pfam	PF02581; TMP-TENI; 1;
TIGRFAMs	TIGR00693; thiE; 1;
PIRSF	PIRSF000512; TMP_PPase_Cyanobac_prd; 1;

Thiamine biosynthesis, Transferase.

Magnesium, Metal-binding.

GO:0000287; Molecular function: magnesium ion binding.
GO:0004789; Molecular function: thiamine-phosphate diphosphorylase activity.
GO:0009228; Biological process: thiamine biosynthetic process.

From: THIE_NOSS1 (Q8YX72)
Key		From		To		Description		Condition		FTGroup
REGION		Nter		129		Unknown
REGION		130		Cter		Thiamine-phosphate synthase
REGION		177		181		HMP-PP binding (By similarity)		Q-x-R-x-K
REGION (Optional)		274		276		THZ-P binding (By similarity)		T-x-T
METAL		210		210		Magnesium (By similarity)		[DN]		1
METAL		229		229		Magnesium (By similarity)		[DE]		1
BINDING		209		209		HMP-PP (By similarity)		N
BINDING		248		248		HMP-PP (By similarity)		S
BINDING		277		277		HMP-PP (By similarity)		K
BINDING		304		304		THZ-P; via amide nitrogen (By similarity)		G

Size range:	341-353 amino acids
Related UniRules:	MF_00097 (THIE)
Template:	Q8YX72 (THIE_NOSS1)
Scope:	Bacteria; Cyanobacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	Unknown N-terminal domain in Cyanobacteria. All other bacteria without this domain belong to family MF_00097.