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HAMAP rule MF_01338

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General rule information [?]

Accession MF_01338
Dates 5-AUG-2005 (Created)
17-FEB-2023 (Last updated, Version 50)
Name RuBisCO_L_type1
Scope(s) Bacteria
Cyanobacteriota
Pseudomonadota
Actinomycetota
Verrucomicrobiota
Plastid
Template(s) P00876 (RBL_TOBAC); P0C2C2 (RBL1C_CUPNE); P00880 (RBL_SYNP6); P00877 (RBL_CHLRE); P0C512 (RBL_ORYSJ); P00875 (RBL_SPIOL); F4CQ77 (RBL_PSEUX); Q31NB3 (RBL_SYNE7); [ Recover all ]
Triggered by HAMAP; MF_01338 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RBL
case <OG:Chloroplast> and <OC:Viridiplantae>
Protein name RecName: Full=Ribulose bisphosphate carboxylase large chain;
                 Short=RuBisCO large subunit;
                 EC=4.1.1.39;
                 Flags: Precursor;
Gene name Name=rbcL;
else case <OG:Chloroplast> and not <OC:Viridiplantae>
Protein name RecName: Full=Ribulose bisphosphate carboxylase large chain;
                 Short=RuBisCO large subunit;
                 EC=4.1.1.39;
Gene name Name=rbcL;
else case <OC:Cyanobacteriota>
Protein name RecName: Full=Ribulose bisphosphate carboxylase large chain;
                 Short=RuBisCO large subunit;
                 EC=4.1.1.39;
Gene name Name=cbbL; Synonyms=rbcL;
else case not <OC:Cyanobacteriota> and not <OG:Chloroplast>
Protein name RecName: Full=Ribulose bisphosphate carboxylase large chain;
                 Short=RuBisCO large subunit;
                 EC=4.1.1.39;
Gene name Name=cbbL;
end case

Comments [?]

case <OG:Chloroplast> or <OC:Cyanobacteriota>
FUNCTIONRuBisCO catalyzes two reactions: the carboxylation of D- ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate in the photorespiration process. Both reactions occur simultaneously and in competition at the same active site.
else case not <OC:Cyanobacteriota> and not <OG:Chloroplast>
FUNCTIONRuBisCO catalyzes two reactions: the carboxylation of D- ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.
end case
CATALYTIC ACTIVITY Reaction=2 (2R)-3-phosphoglycerate + 2 H(+) = CO2 + D-ribulose 1,5- bisphosphate + H2O; Xref=Rhea:RHEA:23124, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:57870, ChEBI:CHEBI:58272; EC=4.1.1.39;
CATALYTIC ACTIVITY Reaction=D-ribulose 1,5-bisphosphate + O2 = (2R)-3-phosphoglycerate + 2-phosphoglycolate + 2 H(+); Xref=Rhea:RHEA:36631, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:57870, ChEBI:CHEBI:58033, ChEBI:CHEBI:58272;
case <FTGroup:1>
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Note=Binds 1 Mg(2+) ion per subunit.;
end case
case <FT:18>
SUBUNITHeterohexadecamer of 8 large chains and 8 small chains; disulfide-linked. The disulfide link is formed within the large subunit homodimers.
else
SUBUNITHeterohexadecamer of 8 large chains and 8 small chains.
end case
case <OC:Cyanobacteriota>
SUBCELLULAR LOCATIONCarboxysome.
end case
case <OG:Chloroplast>
SUBCELLULAR LOCATIONPlastid, chloroplast.
end case
case <FT:18>
PTMThe disulfide bond which can form in the large chain dimeric partners within the hexadecamer appears to be associated with oxidative stress and protein turnover.
end case
MISCELLANEOUSThe basic functional RuBisCO is composed of a large chain homodimer in a 'head-to-tail' conformation. In form I RuBisCO this homodimer is arranged in a barrel-like tetramer with the small subunits forming a tetrameric 'cap' on each end of the 'barrel'.
SIMILARITYBelongs to the RuBisCO large chain family. Type I subfamily.

Keywords [?]

case <FT:15>
Acetylation
end case
case <OC:Cyanobacteriota>
Carboxysome
end case
Calvin cycle
Carbon dioxide fixation
case <FT:18>
Disulfide bond
end case
Lyase
case <FTGroup:1>
Magnesium
Metal-binding
end case
case <FT:16>
Methylation
end case
Monooxygenase
Oxidoreductase
case <OG:Chloroplast> or <OC:Cyanobacteriota>
Photorespiration
end case
case <OG:Chloroplast> or <Property:PHOTOSYN>
Photosynthesis
end case

Gene Ontology [?]

case <FTGroup:1>
GO:0000287; Molecular function:magnesium ion binding
end case
GO:0015977; Biological process:carbon fixation
GO:0016984; Molecular function:ribulose-bisphosphate carboxylase activity
GO:0019253; Biological process:reductive pentose-phosphate cycle
case <OCellular component:Cyanobacteriota>
GO:0031470; Cellular component:carboxysome
end case
case <OG:Chloroplast>
GO:0009507; Cellular component:chloroplast
end case

Cross-references [?]

PROSITE PS00157; RUBISCO_LARGE; 1;
Pfam PF00016; RuBisCO_large; 1;
Pfam PF02788; RuBisCO_large_N; 1;

Features [?]

From: RBL_TOBAC (P00876)
Key From To Description Tag Condition FTGroup
case <OC:Viridiplantae>
PROPEP 1 2
CHAIN 3 Cter /note="Ribulose bisphosphate carboxylase large chain"
end case
SITE 334 334 /note="Transition state stabilizer" K
ACT_SITE 175 175 /note="Proton acceptor" K
ACT_SITE 294 294 /note="Proton acceptor" H
BINDING 201 201 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/note="via carbamate group"		 K 1
BINDING 203 203 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 D 1
BINDING 204 204 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"		 E 1
BINDING 123 123 /ligand="substrate"
/note="in homodimeric partner"		 N
BINDING 173 173 /ligand="substrate" T
BINDING 177 177 /ligand="substrate" K
BINDING 295 295 /ligand="substrate" R
BINDING 327 327 /ligand="substrate" H
BINDING 379 379 /ligand="substrate" S
case <OC:Viridiplantae>
MOD_RES 3 3 /note="N-acetylproline" P
MOD_RES 14 14 /note="N6,N6,N6-trimethyllysine" K
end case
MOD_RES 201 201 /note="N6-carboxylysine" K
DISULFID 247 247 /note="Interchain; in linked form" C

Additional information [?]

Size range 463-501 amino acids
Related rules MF_01133
MF_01339
Fusion Nter: None Cter: None



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