HAMAP

Accession	MF_01349
Dates	29-MAY-2006 (Created) 25-NOV-2009 (Last updated, Version 13)

Data class

Protein

Predictors

HAMAP; MF_01349; [distribution of match scores in UniProtKB];[seed alignment for MF_01349]

Names

PanCY

Identifier

PANCY

Protein name

RecName: Full=Bifunctional pantoate ligase/cytidylate kinase; RecName: Full=Pantothenate synthetase; Short=PS; EC=6.3.2.1; AltName: Full=Pantoate--beta-alanine ligase; AltName: Full=Pantoate-activating enzyme; RecName: Full=Cytidylate kinase; Short=CK; EC=2.7.4.14; AltName: Full=Cytidine monophosphate kinase; Short=CMP kinase;

Gene name

panC/cmk

FUNCTION: Catalyzes the condensation of pantoate with beta-alanine in an ATP-dependent reaction via a pantoyl-adenylate intermediate (By similarity).

FUNCTION: Displays a CMP kinase activity (By similarity).

CATALYTIC ACTIVITY: ATP + (R)-pantoate + beta-alanine = AMP + diphosphate + (R)-pantothenate.

CATALYTIC ACTIVITY: ATP + (d)CMP = ADP + (d)CDP.

PATHWAY: Cofactor biosynthesis; (R)-pantothenate biosynthesis; (R)-pantothenate from (R)-pantoate and beta-alanine: step 1/1.

SUBCELLULAR LOCATION: Cytoplasm (By similarity).

SIMILARITY: In the N-terminal section; belongs to the pantothenate synthetase family.

SIMILARITY: In the C-terminal section; belongs to the cytidylate kinase family. Type 1 subfamily.

Pfam	PF02224; Cytidylate_kin; 1; PF02569; Pantoate_ligase; 1;
TIGRFAMs	TIGR00017; cmk; 1; TIGR00018; panC; 1; TIGR00125; cyt_tran_rel; 1;

Cytoplasm, ATP-binding, Kinase, Ligase, Multifunctional enzyme, Nucleotide-binding, Pantothenate biosynthesis, Transferase.

GO:0004592; Molecular function: pantoate-beta-alanine ligase activity.
GO:0005524; Molecular function: ATP binding.
GO:0004127; Molecular function: cytidylate kinase activity.
GO:0006220; Biological process: pyrimidine nucleotide metabolic process.
GO:0015940; Biological process: pantothenate biosynthetic process.
GO:0005737; Cellular component: cytoplasm.

From: PANCY_SYNY3 (Q55074)
Key		From		To		Description		Condition		FTGroup
NP_BIND		30		37		ATP (By similarity)		M-x(3)-H-x(2)-H
NP_BIND		150		153		ATP (By similarity)		G-x(2)-D
NP_BIND		187		190		ATP (By similarity)		x(3)-R
REGION		Nter		283		Pantoate--beta-alanine ligase
REGION		284		Cter		Cytidylate kinase
ACT_SITE		37		37		Proton donor (By similarity)		H
BINDING		61		61		Beta-alanine (By similarity)		Q
BINDING		61		61		Pantoate (By similarity)		Q
BINDING		156		156		Pantoate (By similarity)		Q
BINDING (Optional)		179		179		ATP; via amide nitrogen and carbonyl oxygen (By similarity)		[VLIGA]

Size range:	480-542 amino acids
Related UniRules:	MF_00158 (PANC); MF_00238 (KCY); MF_00239 (KCY)
Template:	Q55074 (PANCY_SYNY3); P0A6I0 (KCY_ECOLI): [Recover all]
Scope:	Bacteria; Cyanobacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	None
Comments:	This fusion protein seems only to exist in Cyanobacteria; in other bacteria the 2 enzymes are encoded by separate genes (panC, MF_00158 and cmk, MF_00238) that are not usually in close proximity.