HAMAP rule MF_01356

General rule information [?]

Accession	MF_01356
Dates	17-NOV-2008 (Created) 28-JUN-2024 (Last updated, Version 33)
Name	NDH1_NuoB
Scope(s)	Bacteria Plastid
Template(s)	Q56218 (NQO6_THET8); [ Recover all ]
Triggered by	HAMAP; MF_01356 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

case <OG:Chloroplast>
Identifier	NDHK
Protein name	RecName: Full=NAD(P)H-quinone oxidoreductase subunit K, chloroplastic; EC=7.1.1.-; AltName: Full=NAD(P)H dehydrogenase subunit K; AltName: Full=NADH-plastoquinone oxidoreductase subunit K;
Gene name	Name=ndhK;
else case <OC:Cyanobacteriota>
Identifier	NDHK
Protein name	RecName: Full=NAD(P)H-quinone oxidoreductase subunit K; EC=7.1.1.-; AltName: Full=NAD(P)H dehydrogenase I subunit K; AltName: Full=NDH-1 subunit K; Short=NDH-K;
Gene name	Name=ndhK;
else
Identifier	NUOB
Protein name	RecName: Full=NADH-quinone oxidoreductase subunit B; EC=7.1.1.-; AltName: Full=NADH dehydrogenase I subunit B; AltName: Full=NDH-1 subunit B;
Gene name	Name=nuoB;
end case

Comments [?]

case <OG:Chloroplast>
FUNCTION	NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient.
else case <OC:Cyanobacteriota>
FUNCTION	NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient. Cyanobacterial NDH-1 also plays a role in inorganic carbon-concentration.
else case <OC:Actinomycetota> or <OC:Bacteroidota> or <OC:Chlorobiota> or <OC:Deinococcota> or <OC:Bacillota>
FUNCTION	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
else
FUNCTION	NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient.
end case
case <OG:Chloroplast> or <OC:Cyanobacteriota>
CATALYTIC ACTIVITY	Reaction=a plastoquinone + (n+1) H(+)(in) + NADH = a plastoquinol + n H(+)(out) + NAD(+); Xref=Rhea:RHEA:42608, Rhea:RHEA-COMP:9561, Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:62192;
CATALYTIC ACTIVITY	Reaction=a plastoquinone + (n+1) H(+)(in) + NADPH = a plastoquinol + n H(+)(out) + NADP(+); Xref=Rhea:RHEA:42612, Rhea:RHEA-COMP:9561, Rhea:RHEA-COMP:9562, ChEBI:CHEBI:15378, ChEBI:CHEBI:17757, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:62192;
else
CATALYTIC ACTIVITY	Reaction=a quinone + 5 H(+)(in) + NADH = a quinol + 4 H(+)(out) + NAD(+); Xref=Rhea:RHEA:57888, ChEBI:CHEBI:15378, ChEBI:CHEBI:24646, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:132124;
end case
case <FTGroup:1>
COFACTOR	Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster.;
end case
case <OG:Chloroplast>
SUBUNIT	NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus.
else case <OC:Cyanobacteriota>
SUBUNIT	NDH-1 can be composed of about 15 different subunits; different subcomplexes with different compositions have been identified which probably have different functions.
else case <OC:Enterobacterales> or <OC:Shewanellaceae> or <OC:Pseudomonadaceae>
SUBUNIT	NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex.
else case <OC:Deinococci>
SUBUNIT	NDH-1 is composed of 15 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.
else
SUBUNIT	NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex.
end case
case <OG:Chloroplast>
SUBCELLULAR LOCATION	Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side.
else case <OC:Cyanobacteriota> and not <OC:Gloeobacter>
SUBCELLULAR LOCATION	Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side.
else case <OC:Gloeobacter>
SUBCELLULAR LOCATION	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
else case not defined <Property:Membrane> or <Property:Membrane=1>
SUBCELLULAR LOCATION	Cell membrane; Peripheral membrane protein; Cytoplasmic side.
else case <Property:Membrane=2>
SUBCELLULAR LOCATION	Cell inner membrane; Peripheral membrane protein; Cytoplasmic side.
end case
SIMILARITY	Belongs to the complex I 20 kDa subunit family.

Keywords [?]

case <FTGroup:1>
4Fe-4S
Iron
Iron-sulfur
Metal-binding
end case
NAD
case <OG:Chloroplast> or <OC:Cyanobacteriota>
NADP
Plastoquinone
else case not <OC:Actinomycetota> and not <OC:Bacteroidota> and not <OC:Chlorobiota> and not <OC:Deinococcota> and not <OC:Bacillota>
Ubiquinone
end case
Quinone
Membrane
<a href="https://www.uniprot.org/keywords/KW-1278">Translocase</a>
Transport
case <OG:Chloroplast> or <OC:Cyanobacteriota> and not <OC:Gloeobacter>
Thylakoid
else case <OC:Gloeobacter>
Cell membrane
Cell inner membrane
else case not defined <Property:Membrane> or <Property:Membrane=1>
Cell membrane
else case <Property:Membrane=2>
Cell membrane
Cell inner membrane
end case

Gene Ontology [?]

GO:0005506; Molecular function:iron ion binding
case <OG:Chloroplast> or <OCellular component:Cyanobacteriota>
GO:0016655; Molecular function:oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor
GO:0019684; Biological process:photosynthesis, light reaction
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0050136; Molecular function:NADH:ubiquinone reductase (non-electrogenic) activity
end case
case <OCellular component:Cyanobacteriota> and not <OC:Gloeobacter>
GO:0042651; Cellular component:thylakoid membrane
else case <OG:Chloroplast>
GO:0009535; Cellular component:chloroplast thylakoid membrane
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0005886; Cellular component:plasma membrane
end case

Cross-references [?]

PROSITE	PS01150; COMPLEX1_20K; 1;
Pfam	PF01058; Oxidored_q6; 1;
NCBIfam	TIGR01957; NuoB_fam; 1;

Features [?]

From: NQO6_THET8 (Q56218)

Key

From

Description

Tag

Condition

FTGroup

BINDING

/ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"

BINDING

/ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"

BINDING

111

/ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"

BINDING

140

/ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"

Additional information [?]

Size range	158-281 amino acids
Related rules	None
Fusion	Nter: None Cter: MF_01359 (nuoCD)
Comments	14 proteins form the NDH-1 complex in most bacteria, there are at least 15 subunits in cyanobacteria and chloroplasts. PARDE and THET8 are annotated with another nomenclature. In the Geobacter there is also a fusion with nuoCD.

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