Home Proteomes Families Hamap-Scan Documents Downloads Links
HAMAP annotation rule: MF_01356
Send feedback new

General rule information

Accession MF_01356
Dates 17-NOV-2008 (Created)
6-OCT-2011 (Last updated, Version 10)
Data class Protein
Predictors HAMAP; MF_01356; [distribution of match scores in UniProtKB];[seed alignment for MF_01356]
Names NDH1_NuoB


Propagated annotation

Identifier, protein and gene names

case <OG:Chloroplast>
Identifier NDHK
Protein name
RecName: Full=NAD(P)H-quinone oxidoreductase subunit K, chloroplastic;
EC=1.6.5.-;
AltName: Full=NAD(P)H dehydrogenase subunit K;
AltName: Full=NADH-plastoquinone oxidoreductase subunit K;
Gene name ndhK

else case <OC:Cyanobacteria>
Identifier NDHK
Protein name
RecName: Full=NAD(P)H-quinone oxidoreductase subunit K;
EC=1.6.5.-;
AltName: Full=NAD(P)H dehydrogenase I subunit K;
AltName: Full=NDH-1 subunit K;
Short=NDH-K;
Gene name ndhK

else
Identifier NUOB
Protein name
RecName: Full=NADH-quinone oxidoreductase subunit B;
EC=1.6.99.5;
AltName: Full=NADH dehydrogenase I subunit B;
AltName: Full=NDH-1 subunit B;
Gene name nuoB
end case

Comments

case <OG:Chloroplast>
FUNCTION: NDH shuttles electrons from NAD(P)H:plastoquinone, via FMN and iron-sulfur (Fe-S) centers, to quinones in the photosynthetic chain and possibly in a chloroplast respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient (By similarity).

else case <OC:Cyanobacteria>
FUNCTION: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone. Couples the redox reaction to proton translocation, and thus conserves the redox energy in a proton gradient. Cyanobacterial NDH-1 also plays a role in inorganic carbon-concentration (By similarity).

else case <OC:Actinobacteria> or <OC:Bacteroidetes> or <OC:Chlorobi> or <OC:Deinococcus-Thermus> or <OC:Firmicutes>
FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).

else
FUNCTION: NDH-1 shuttles electrons from NADH, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cytoplasmic membrane), and thus conserves the redox energy in a proton gradient (By similarity).
end case


case <OG:Chloroplast> or <OC:Cyanobacteria>
CATALYTIC ACTIVITY: NAD(P)H + plastoquinone = NAD(P)(+) + plastoquinol.

else
CATALYTIC ACTIVITY: NADH + quinone = NAD(+) + quinol.
end case


case <FTGroup:1>
COFACTOR: Binds 1 4Fe-4S cluster (By similarity).
end case


case <OG:Chloroplast>
SUBUNIT: NDH is composed of at least 16 different subunits, 5 of which are encoded in the nucleus (By similarity).

else case <OC:Cyanobacteria>
SUBUNIT: NDH-1 can be composed of about 15 different subunits; different subcomplexes with different compositions have been identified which probably have different functions (By similarity).

else case <OC:Enterobacteriaceae> or <OC:Shewanellaceae> or <OC:Pseudomonadaceae>
SUBUNIT: NDH-1 is composed of 13 different subunits. Subunits NuoB, CD, E, F, and G constitute the peripheral sector of the complex (By similarity).

else case <OC:Deinococci>
SUBUNIT: NDH-1 is composed of 15 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex (By similarity).

else
SUBUNIT: NDH-1 is composed of 14 different subunits. Subunits NuoB, C, D, E, F, and G constitute the peripheral sector of the complex (By similarity).
end case


case <OG:Chloroplast>
SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side (By similarity).

else case <OC:Cyanobacteria> and not <OC:Gloeobacter>
SUBCELLULAR LOCATION: Cellular thylakoid membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).

else case <OC:Gloeobacter>
SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).

else case not defined <Property:Membrane> or <Property:Membrane=1>
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).

else case <Property:Membrane=2>
SUBCELLULAR LOCATION: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side (By similarity).
end case

SIMILARITY: Belongs to the complex I 20 kDa subunit family.
Cross-references
PROSITE PS01150; COMPLEX1_20K; 1;
Pfam PF01058; Oxidored_q6; 1;
TIGRFAMs TIGR01957; NuoB_fam; 1;
Keywords

case <FTGroup:1>
4Fe-4S, Iron, Iron-sulfur, Metal-binding.
end case

NAD.

case <OG:Chloroplast> or <OC:Cyanobacteria>
NADP, Plastoquinone.

else case not <OC:Actinobacteria> and not <OC:Bacteroidetes> and not <OC:Chlorobi> and not <OC:Deinococcus-Thermus> and not <OC:Firmicutes>
Ubiquinone.
end case

Oxidoreductase, Quinone, Membrane, Transport.

case <OG:Chloroplast> or <OC:Cyanobacteria> and not <OC:Gloeobacter>
Thylakoid.

else case <OC:Gloeobacter>
Cell membrane, Cell inner membrane.

else case not defined <Property:Membrane> or <Property:Membrane=1>
Cell membrane.

else case <Property:Membrane=2>
Cell membrane, Cell inner membrane.
end case

Gene Ontology
GO:0005506; Molecular function: iron ion binding.
GO:0055114; Biological process: oxidation-reduction process.
GO:0019684; Biological process: photosynthesis, light reaction.

case <OG:Chloroplast> or <OC:Cyanobacteria>
GO:0016655; Molecular function: oxidoreductase activity, acting on NADH or NADPH, quinone or similar compound as acceptor.

else
GO:0050136; Molecular function: NADH dehydrogenase (quinone) activity.
end case


case <OC:Cyanobacteria> and not <OC:Gloeobacter>
GO:0042651; Cellular component: thylakoid membrane.

else case <OG:Chloroplast>
GO:0009535; Cellular component: chloroplast thylakoid membrane.

else
GO:0005886; Cellular component: plasma membrane.
end case

Features
From: NQO6_THET8 (Q56218)
Key     From     To       Description   Condition   FTGroup
METAL     45     45       Iron-sulfur (4Fe-4S) (Potential)   C   1
METAL     46     46       Iron-sulfur (4Fe-4S) (Potential)   C   1
METAL     111     111       Iron-sulfur (4Fe-4S) (Potential)   C   1
METAL     140     140       Iron-sulfur (4Fe-4S) (Potential)   C   1


Additional information

Size range: 158-281 amino acids
Related UniRules: None
Template: Q56218 (NQO6_THET8)
Scope: Bacteria
Plastid
Fusion: Nter: None; Cter: MF_01359 (nuoCD)
Duplicate: in ACAM1, KORVE, ACICJ, ANADE, ANADF, ANASK, AZOSB, BURMA, BURM7, BURM9, BURMS, BURP0, BURP1, BURP6, BURPS, CHLAA, CHLAD, CHLSY, CHLT3, CHRVO, CYAP4, CYTH3, GEOBB, GEOMG, GEOSL, GEOUR, GLOVI, GLUDA, HERA2, NITMU, NITOC, OPITP, PELPD, RHIME, RHIE6, RHIEC, RHOP5, RHOPA, RHOPB, RHOPS, RHOPT, RHOS1, RHOS4, RHOS5, RHOSK, ROSCS, ROSS1, SALAI, SALTO, SOLUE, SORC5, STRAW, STRCO, STRGG, SYMTH, SYNY3, THEYD
Plasmid encoded: in ACAM1, RHIME
Comments: 14 proteins form the NDH-1 complex in most bacteria, there are at least 15 subunits in cyanobacteria and chloroplasts. PARDE and THET8 are annotated with another nomenclature. In the Geobacter there is also a fusion with nuoCD.

View rule in raw text format (no links)



UniProtKB rule member sequences