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HAMAP rule MF_01446

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General rule information [?]

Accession MF_01446
Dates 13-AUG-2007 (Created)
1-JUN-2023 (Last updated, Version 23)
Name Kae1
Scope(s) Archaea
Template(s) Q9UXT7 (KAE1_PYRAB); P36132 (KAE1_YEAST); [ Recover all ]
Triggered by
case c? <OC:Archaea>
HAMAP; MF_01446 (Get profile general information and statistics)
end case

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier KAE1
Protein name RecName: Full=tRNA N6-adenosine threonylcarbamoyltransferase;
                 EC=2.3.1.234;
AltName: Full=N6-L-threonylcarbamoyladenine synthase;
                 Short=t(6)A synthase;
AltName: Full=tRNA threonylcarbamoyladenosine biosynthesis protein Kae1;
AltName: Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1;
Gene name Name=kae1;

Comments [?]

case <OC:Euryarchaeota>
FUNCTIONRequired for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Kae1 likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity.
SUBUNITMonomer. Component of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.
else
FUNCTIONRequired for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37.
end case
CATALYTIC ACTIVITY Reaction=adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA; Xref=Rhea:RHEA:37059, Rhea:RHEA-COMP:10162, Rhea:RHEA-COMP:10163, ChEBI:CHEBI:15378, ChEBI:CHEBI:73682, ChEBI:CHEBI:74411, ChEBI:CHEBI:74418, ChEBI:CHEBI:456215; EC=2.3.1.234;
COFACTOR Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Note=Binds 1 Fe(2+) ion per subunit.;
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYBelongs to the KAE1 / TsaD family.

Keywords [?]

Acyltransferase
Cytoplasm
Iron
Metal-binding
Transferase
tRNA processing

Gene Ontology [?]

GO:0005506; Molecular function:iron ion binding
GO:0016747; Molecular function:acyltransferase activity, transferring groups other than amino-acyl groups
GO:0002949; Biological process:tRNA threonylcarbamoyladenosine modification
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

PROSITE PS01016; GLYCOPROTEASE; 1;
Pfam PF00814; Peptidase_M22; 1;
PRINTS PR00789; OSIALOPTASE; 1;
NCBIfam TIGR03722; Arch_KAE1; 1;
NCBIfam TIGR00329; Gcp_kae1; 1;

Features [?]

From: KAE1_PYRAB (Q9UXT7)
Key From To Description Tag Condition FTGroup
BINDING 127 131 /ligand="substrate" x-x-[SA]-G-[GA]
BINDING 107 107 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"		 H
BINDING 111 111 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"		 H
BINDING 127 127 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"		 Y
BINDING 285 285 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"		 D
BINDING 159 159 /ligand="substrate" D
BINDING 172 172 /ligand="substrate" G
BINDING 176 176 /ligand="substrate" [ED]
BINDING 257 257 /ligand="substrate" [NS]

Additional information [?]

Size range 314-363 amino acids
Related rules MF_01447
Fusion Nter: None Cter: None
Comments Was originally (PubMed:17766251) thought to have endonuclease activity, but it could not be confirmed with orthologs purified from M. jannaschii (PubMed:18951093) and S. cerevisiae (PubMed:21183954). Some Archaea contain a kinase domain in the C-terminus. These sequences are represented in the MF_01447 family rule.



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