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HAMAP rule MF_01447

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General rule information [?]

Accession MF_01447
Dates 13-AUG-2007 (Created)
1-JUN-2023 (Last updated, Version 23)
Name Kae1_Bud32_arch
Scope(s) Archaea
Euryarchaeota
Template(s) Q58530 (KAE1B_METJA); P36132 (KAE1_YEAST); [ Recover all ]
Triggered by HAMAP; MF_01447 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier KAE1B
Protein name RecName: Full=Probable bifunctional tRNA threonylcarbamoyladenosine biosynthesis protein;
                 Includes:
RecName: Full=tRNA N6-adenosine threonylcarbamoyltransferase;
                 EC=2.3.1.234;
AltName: Full=tRNA threonylcarbamoyladenosine biosynthesis protein Kae1;
AltName: Full=t(6)A37 threonylcarbamoyladenosine biosynthesis protein Kae1;
                 Includes:
RecName: Full=Serine/threonine-protein kinase Bud32;
                 EC=2.7.11.1;

Comments [?]

FUNCTIONRequired for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. Is a component of the KEOPS complex that is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. The Kae1 domain likely plays a direct catalytic role in this reaction. The Bud32 domain probably displays kinase activity that regulates Kae1 function.
CATALYTIC ACTIVITY Reaction=adenosine(37) in tRNA + L-threonylcarbamoyladenylate = AMP + H(+) + N(6)-L-threonylcarbamoyladenosine(37) in tRNA; Xref=Rhea:RHEA:37059, Rhea:RHEA-COMP:10162, Rhea:RHEA-COMP:10163, ChEBI:CHEBI:15378, ChEBI:CHEBI:73682, ChEBI:CHEBI:74411, ChEBI:CHEBI:74418, ChEBI:CHEBI:456215; EC=2.3.1.234;
CATALYTIC ACTIVITY Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl- [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA- COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616, ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CATALYTIC ACTIVITY Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L- threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060, Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013, ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216; EC=2.7.11.1;
COFACTOR Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Note=Binds 1 Fe(2+) ion per subunit.;
SUBUNITComponent of the KEOPS complex that consists of Kae1, Bud32, Cgi121 and Pcc1; the whole complex dimerizes.
SUBCELLULAR LOCATIONCytoplasm.
SIMILARITYIn the N-terminal section; belongs to the KAE1 / TsaD family.
SIMILARITYIn the C-terminal section; belongs to the protein kinase superfamily. Tyr protein kinase family. BUD32 subfamily.

Keywords [?]

Acyltransferase
ATP-binding
Cytoplasm
Kinase
Nucleotide-binding
Serine/threonine-protein kinase
Transferase
tRNA processing
Iron
Metal-binding
Multifunctional enzyme

Gene Ontology [?]

GO:0005506; Molecular function:iron ion binding
GO:0005524; Molecular function:ATP binding
GO:0004712; Molecular function:protein serine/threonine/tyrosine kinase activity
GO:0016747; Molecular function:acyltransferase activity, transferring groups other than amino-acyl groups
GO:0002949; Biological process:tRNA threonylcarbamoyladenosine modification
GO:0005737; Cellular component:cytoplasm

Cross-references [?]

PROSITE PS01016; GLYCOPROTEASE; 1;
PROSITE PS00107; PROTEIN_KINASE_ATP; 1;
PROSITE PS50011; PROTEIN_KINASE_DOM; 1;
PROSITE PS00109; PROTEIN_KINASE_TYR; 1;
Pfam PF00814; Peptidase_M22; 1;
Pfam PF00069; Pkinase; 1;
Pfam PF01163; RIO1; 1;
PRINTS PR00789; OSIALOPTASE; 1;
NCBIfam TIGR03724; Arch_bud32; 1;
NCBIfam TIGR03722; Arch_KAE1; 1;
NCBIfam TIGR00329; Gcp_kae1; 1;
PIRSF PIRSF036401; Gcp_STYKS; 1;

Features [?]

From: KAE1B_METJA (Q58530)
Key From To Description Tag Condition FTGroup
DOMAIN 333 Cter /note="Protein kinase"
BINDING 339 347 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
REGION Nter 323 /note="Kae1"
ACT_SITE 451 451 /note="Proton acceptor; for kinase activity" D
BINDING 127 131 /ligand="L-threonylcarbamoyladenylate"
/ligand_id="ChEBI:CHEBI:73682"		 x-x-S-G-[GA]
BINDING 106 106 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"		 H
BINDING 110 110 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"		 H
BINDING 127 127 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"		 Y
BINDING 284 284 /ligand="Fe cation"
/ligand_id="ChEBI:CHEBI:24875"		 D
BINDING 159 159 /ligand="L-threonylcarbamoyladenylate"
/ligand_id="ChEBI:CHEBI:73682"		 D
BINDING 172 172 /ligand="L-threonylcarbamoyladenylate"
/ligand_id="ChEBI:CHEBI:73682"		 G
BINDING 176 176 /ligand="L-threonylcarbamoyladenylate"
/ligand_id="ChEBI:CHEBI:73682"		 E
BINDING 256 256 /ligand="L-threonylcarbamoyladenylate"
/ligand_id="ChEBI:CHEBI:73682"		 N
BINDING 360 360 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"		 K

Additional information [?]

Size range 520-548 amino acids
Related rules MF_01446
Fusion Nter: None Cter: None



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