HAMAP logo

HAMAP rule MF_01452

Send feedback

General rule information [?]

Accession MF_01452
Dates 4-JUN-2009 (Created)
10-NOV-2023 (Last updated, Version 20)
Name AddB_type1
Scope(s) Bacteria
Bacillota
Template(s) P23477 (ADDB_BACSU); [ Recover all ]
Triggered by HAMAP; MF_01452 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier ADDB
RecName: Full=ATP-dependent helicase/deoxyribonuclease subunit B;<br /> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;EC=<a href="https://enzyme.expasy.org/EC/3.1.-.-">3.1.-.-</a>;<br /> AltName: Full=ATP-dependent helicase/nuclease subunit AddB;
Gene name Name=addB;

Comments [?]

FUNCTIONThe heterodimer acts as both an ATP-dependent DNA helicase and an ATP-dependent, dual-direction single-stranded exonuclease. Recognizes the chi site generating a DNA molecule suitable for the initiation of homologous recombination. The AddB subunit has 5' -> 3' nuclease activity but not helicase activity.
case <FTGroup:1>
COFACTOR Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883; Note=Binds 1 [4Fe-4S] cluster.;
end case
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
SUBUNITHeterodimer of AddA and AddB.
MISCELLANEOUSDespite having conserved helicase domains, this subunit does not have helicase activity.
SIMILARITYBelongs to the helicase family. AddB/RexB type 1 subfamily.

Keywords [?]

ATP-binding
DNA damage
DNA repair
DNA-binding
Exonuclease
<a href="https://www.uniprot.org/keywords/KW-0347">Helicase</a>
Hydrolase
Nuclease
Nucleotide-binding
case <FTGroup:1>
4Fe-4S
Iron
Iron-sulfur
Metal-binding
end case

Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
GO:0008409; Molecular function:5'-3' exonuclease activity
GO:0003690; Molecular function:double-stranded DNA binding
case <FTGroup:1>
GO:0051536; Molecular function:iron-sulfur cluster binding
end case
GO:0000724; Biological process:double-strand break repair via homologous recombination

Cross-references [?]

Pfam PF21445; ADDB_N; 1;
Pfam PF12705; PDDEXK_1; 1;
PROSITE PS51198; UVRD_HELICASE_ATP_BIND; 0-1;
PROSITE PS51217; UVRD_HELICASE_CTER; 0-1;
NCBIfam TIGR02773; AddB_Gpos; 1;

Features [?]

From: ADDB_BACSU (P23477)
Key From To Description Tag Condition FTGroup
BINDING 801 801 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
C 1
BINDING 1121 1121 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
C 1
BINDING 1124 1124 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
C 1
BINDING 1130 1130 /ligand="[4Fe-4S] cluster"
/ligand_id="ChEBI:CHEBI:49883"
C 1

Additional information [?]

Size range 1134-1224 amino acids
Related rules MF_01453
Fusion Nter: None Cter: None
Comments Two related subfamiles of this protein exist; addB in Bacillus and Staphylococcus (this family), and rexB in Lactobacillus and Streptococcus (MF_01453). In most Lactobacillales there is no 4Fe-4S center; this has been suggested (PubMed:19129187) to occur in organisms which inhabit a low-iron environment. Not all members match the uvrD_helicase profiles (PS51198 and PS51217).



View rule in raw text format (no links)