HAMAP rule MF_01470

General rule information [?]

Accession	MF_01470
Dates	26-MAR-2012 (Created) 7-DEC-2023 (Last updated, Version 32)
Name	Cas1
Scope(s)	Bacteria Archaea
Template(s)	Q02ML7 (CAS1_PSEAB); Q46896 (CAS1_ECOLI); [ Recover all ]
Triggered by	HAMAP; MF_01470 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier	CAS1
Protein name	RecName: Full=CRISPR-associated endonuclease Cas1; EC=3.1.-.-;
Gene name	Name=cas1;

Comments [?]

FUNCTION	CRISPR (clustered regularly interspaced short palindromic repeat), is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA). Acts as a dsDNA endonuclease. Involved in the integration of spacer DNA into the CRISPR cassette.
COFACTOR	Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
SUBUNIT	Homodimer, forms a heterotetramer with a Cas2 homodimer.
SIMILARITY	Belongs to the CRISPR-associated endonuclease Cas1 family.

Keywords [?]

Gene Ontology [?]

GO:0004519; Molecular function:endonuclease activity

GO:0046872; Molecular function:metal ion binding

GO:0051607; Biological process:defense response to virus

GO:0043571; Biological process:maintenance of CRISPR repeat elements

Cross-references [?]

Pfam	PF01867; Cas_Cas1; 1;
NCBIfam	TIGR00287; Cas1; 1;

Features [?]

From: CAS1_PSEAB (Q02ML7)

Key

From

Description

Tag

Condition

FTGroup

BINDING

190

/ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"

BINDING

254

/ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"

BINDING

268

/ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"

[DE]

Additional information [?]

Size range	250-437 amino acids
Related rules	None
Fusion	Nter: <Cas4>; <Reverse transcriptase> Cter: None
Comments	Frequently duplicated, there are many subfamiles, this one however is the master family. Sometimes found fused after Cas4 or a reverse transcriptase domain. One of the METRM (Q2FPW6) is missing residues predicted to be involved in metal-binding and is atypical.