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HAMAP annotation rule: MF_01520
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General rule information

Accession MF_01520
Dates 13-AUG-2004 (Created)
4-FEB-2011 (Last updated, Version 22)
Data class Protein
Predictors HAMAP; MF_01520; [distribution of match scores in UniProtKB];[seed alignment for MF_01520]
Names IspDF


Propagated annotation

Identifier, protein and gene names
Identifier ISPDF
Protein name
RecName: Full=Bifunctional enzyme IspD/IspF;
RecName: Full=2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase;
EC=2.7.7.60;
AltName: Full=4-diphosphocytidyl-2C-methyl-D-erythritol synthase;
AltName: Full=MEP cytidylyltransferase;
Short=MCT;
RecName: Full=2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase;
Short=MECDP-synthase;
Short=MECPS;
EC=4.6.1.12;
Gene name ispDF
Comments
FUNCTION: Bifunctional enzyme that catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP) (IspD), and converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP (IspF) (By similarity).
CATALYTIC ACTIVITY: CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.
CATALYTIC ACTIVITY: 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP.
COFACTOR: Divalent metal cations (By similarity).
PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 2/6.
PATHWAY: Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 4/6.
SIMILARITY: In the N-terminal section; belongs to the IspD family.
SIMILARITY: In the C-terminal section; belongs to the IspF family.
Cross-references
Pfam PF01128; IspD; 1;
PF02542; YgbB; 1;
TIGRFAMs TIGR00453; ispD; 1;
TIGR00151; ispF; 1;
PROSITE PS01295; ISPD; 1;
PS01350; ISPF; 1;
Keywords
Isoprene biosynthesis, Lyase, Metal-binding, Multifunctional enzyme, Nucleotidyltransferase, Transferase.
Gene Ontology
GO:0008685; Molecular function: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity.
GO:0050518; Molecular function: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase activity.
GO:0016114; Biological process: terpenoid biosynthetic process.
Features
From: ISPDF_CAMJE (Q9PM68)
Key     From     To       Description   Condition   FTGroup
REGION     Nter     210       2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase      
REGION     211     Cter       2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase      
METAL     217     217       Divalent metal cation (By similarity)   D  
METAL     219     219       Divalent metal cation (By similarity)   H  
METAL     251     251       Divalent metal cation (By similarity)   H  
SITE     16     16       Transition state stabilizer (By similarity)   R  
SITE     23     23       Transition state stabilizer (By similarity)   K  
SITE     139     139       Positions MEP for the nucleophilic attack (By similarity)   [RH]  
SITE     191     191       Positions MEP for the nucleophilic attack (By similarity)   K  
SITE     243     243       Transition state stabilizer (By similarity)   [HDN]  
SITE     342     342       Transition state stabilizer (By similarity)   [TS]  


Additional information

Size range: 359-422 amino acids
Related UniRules: MF_00107 (ISPF); MF_00108 (ISPD)
Template: Q9PM68 (ISPDF_CAMJE); Q46893 (ISPD_ECOLI); P62617 (ISPF_ECOLI): [Recover all]
Scope: Bacteria
Fusion: Nter: None; Cter: None
Duplicate: None
Plasmid encoded: None
Comments: See MF_00108 and MF_00107 for monofunctional ispD and ispF

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UniProtKB rule member sequences