HAMAP

Accession	MF_01541
Dates	22-AUG-2005 (Created) 2-DEC-2011 (Last updated, Version 11)

Data class

Protein

Predictors

HAMAP; MF_01541; [distribution of match scores in UniProtKB];[seed alignment for MF_01541]

Names

CysJ

Identifier

CYSJ

Protein name

RecName: Full=Sulfite reductase [NADPH] flavoprotein alpha-component; Short=SiR-FP; EC=1.8.1.2;

Gene name

cysJ

FUNCTION: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L-cysteine from sulfate. The flavoprotein component catalyzes the electron flow from NADPH -> FAD -> FMN to the hemoprotein component (By similarity).

CATALYTIC ACTIVITY: H(2)S + 3 NADP(+) + 3 H(2)O = sulfite + 3 NADPH.

COFACTOR: Binds 1 FAD per subunit (By similarity).

COFACTOR: Binds 1 FMN per subunit (By similarity).

PATHWAY: Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1.

SUBUNIT: Alpha(8)-beta(8). The alpha component is a flavoprotein, the beta component is a hemoprotein (By similarity).

PROSITE	PS50902; FLAVODOXIN_LIKE; 1; trigger=PRU00088; PS51384; FAD_FR; 1; trigger=PRU00716;
Pfam	PF00667; FAD_binding_1; 1; PF00258; Flavodoxin_1; 1; PF00175; NAD_binding_1; 1;
PRINTS	PR00369; FLAVODOXIN; 1; PR00371; FPNCR; 1;
TIGRFAMs	TIGR01931; CysJ; 1;

Amino-acid biosynthesis, Cysteine biosynthesis, Electron transport, FAD, Flavoprotein, FMN, NADP, Oxidoreductase, Transport.

GO:0004783; Molecular function: sulfite reductase (NADPH) activity.
GO:0000103; Biological process: sulfate assimilation.
GO:0070814; Biological process: hydrogen sulfide biosynthetic process.

From: CYSJ_ECOLI (P38038)
Key		From		To		Description		Condition		FTGroup
NP_BIND (Optional)		70		74		FMN (By similarity)		S-Q-T-G-N
NP_BIND (Optional)		117		122		FMN (By similarity)		S-T-[QHN]-G-[ED]-G
NP_BIND		150		181		FMN (By similarity)
NP_BIND (Optional)		386		389		FAD (By similarity)		R-[LF]-Y-S
NP_BIND (Optional)		420		422		FAD (By similarity)		G-[AS]-S
NP_BIND (Optional)		519		527		NADP (By similarity)		S-[RQ]-D-Q-x-x-K-[IV]-Y
BINDING (Optional)		489		489		NADP (By similarity)		[ND]

Size range:	595-623 amino acids
Related UniRules:	None
Template:	P38038 (CYSJ_ECOLI); P38039 (CYSJ_SALTY): [Recover all]
Scope:	Bacteria; Proteobacteria
Fusion:	Nter: None; Cter: None
Duplicate:	in NEIMB
Plasmid encoded:	None