HAMAP rule MF_01547
General rule information
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Accession | MF_01547 |
Dates | 22-NOV-2005 (Created)
1-JUN-2023 (Last updated, Version 28) |
Name | RNA_methyltr_E |
Scope(s) |
Bacteria Archaea |
Template(s) | P0C0R7 (RLME_ECOLI); [ Recover all ] |
Triggered by |
case c? <OC:Bacteria> or <OC:Archaea>
HAMAP; MF_01547 (Get profile general information and statistics) end case
|
Propagated annotation
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Identifier, protein and gene names
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Identifier | RLME |
Protein name | RecName: Full=Ribosomal RNA large subunit methyltransferase E; EC=2.1.1.166; AltName: Full=23S rRNA Um2552 methyltransferase; AltName: Full=rRNA (uridine-2'-O-)-methyltransferase; |
case <OC:Bacteria> | |
Gene name | Name=rlmE; Synonyms=ftsJ, rrmJ; |
else case <OC:Archaea> | |
Gene name | Name=rlmE; |
end case |
Comments
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FUNCTION | Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit. |
CATALYTIC ACTIVITY | Reaction=S-adenosyl-L-methionine + uridine(2552) in 23S rRNA = 2'-O- methyluridine(2552) in 23S rRNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:42720, Rhea:RHEA-COMP:10202, Rhea:RHEA-COMP:10203, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:65315, ChEBI:CHEBI:74478; EC=2.1.1.166; |
SUBCELLULAR LOCATION | Cytoplasm. |
SIMILARITY | Belongs to the class I-like SAM-binding methyltransferase superfamily. RNA methyltransferase RlmE family. |
Keywords
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Gene Ontology
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GO:0008650; Molecular function:rRNA (uridine-2'-O-)-methyltransferase activity |
GO:0006364; Biological process:rRNA processing |
GO:0005737; Cellular component:cytoplasm |
Cross-references
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PROSITE | PS50926; TRAM; 0-1; |
Pfam | PF01728; FtsJ; 1; |
Pfam | PF01938; TRAM; 0-1; |
PIRSF | PIRSF005461; 23S_rRNA_mtase; 1; |
NCBIfam | TIGR00438; RrmJ; 1; |
Features
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From: RLME_ECOLI (P0C0R7) | ||||||||||||
Key | From | To | Description | Tag | Condition | FTGroup | ||||||
ACT_SITE | 164 | 164 | /note="Proton acceptor" | K | ||||||||
BINDING | 63 | 63 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
G | 1 | |||||||
BINDING | 65 | 65 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[WF] | 1 | |||||||
BINDING | 83 | 83 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
D | 1 | |||||||
BINDING | 99 | 99 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
[DN] | 1 | |||||||
BINDING | 124 | 124 | /ligand="S-adenosyl-L-methionine" /ligand_id="ChEBI:CHEBI:59789" |
D | 1 |
Additional information
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Size range | 189-279 amino acids |
Related rules |
MF_03162 MF_03163 |
Fusion | Nter: None Cter: None |