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HAMAP rule MF_01552

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General rule information [?]

Accession MF_01552
Dates 30-NOV-2005 (Created)
1-JUN-2023 (Last updated, Version 25)
Name RimK
Scope(s) Bacteria
Cyanobacteriota
Planctomycetota
Pseudomonadota
Archaea
Methanomicrobia
Template(s) P0C0U4 (RIMK_ECOLI); [ Recover all ]
Triggered by HAMAP; MF_01552 (Get profile general information and statistics)

Propagated annotation [?]

Identifier, protein and gene names [?]

Identifier RIMK
case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
Protein name RecName: Full=Ribosomal protein bS6--L-glutamate ligase;
                 EC=6.3.2.-;
AltName: Full=Poly-alpha-glutamate synthase;
AltName: Full=Ribosomal protein bS6 modification protein;
else
Protein name RecName: Full=Probable alpha-L-glutamate ligase;
                 EC=6.3.2.-;
end case
Gene name Name=rimK;

Comments [?]

case <OC:Escherichia> or <OC:Shigella> or <OC:Salmonella>
FUNCTIONAn L-glutamate ligase that catalyzes the ATP-dependent post- translational addition of glutamate residues to the C-terminus of ribosomal protein bS6 (RpsF). Is also able to catalyze the synthesis of poly-alpha-glutamate in vitro, via ATP hydrolysis from unprotected glutamate as substrate. The number of glutamate residues added to either RpsF or to poly-alpha-glutamate changes with pH.
end case
case <FTGroup:1>
COFACTOR Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Note=Binds 2 magnesium or manganese ions per subunit.;
end case
SIMILARITYBelongs to the RimK family.

Keywords [?]


Gene Ontology [?]

GO:0005524; Molecular function:ATP binding
case <OCellular component:Escherichia> or <OC:Shigella> or <OC:Salmonella>
GO:0018169; Molecular function:ribosomal S6-glutamic acid ligase activity
GO:0018410; Biological process:C-terminal protein amino acid modification
else; https://www.ebi.ac.uk/QuickGO/term/else
GO:0016881; Molecular function:acid-amino acid ligase activity
end case

Cross-references [?]

PROSITE PS50975; ATP_GRASP; 1;
Pfam PF02222; ATP-grasp; 1;
NCBIfam TIGR00768; RimK_fam; 1;

Features [?]

From: RIMK_ECOLI (P0C0U4)
Key From To Description Tag Condition FTGroup
BINDING 178 179 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
[EDQ]-[YF]
BINDING 211 213 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
R-[SA]-N
BINDING 248 248 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"
D 1
BINDING 248 248 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
/ligand_label="1"
D 1
BINDING 260 260 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="1"
E 1
BINDING 260 260 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="2"
E 1
BINDING 260 260 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
/ligand_label="1"
E 1
BINDING 260 260 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
/ligand_label="2"
E 1
BINDING 262 262 /ligand="Mg(2+)"
/ligand_id="ChEBI:CHEBI:18420"
/ligand_label="2"
N 1
BINDING 262 262 /ligand="Mn(2+)"
/ligand_id="ChEBI:CHEBI:29035"
/ligand_label="2"
N 1
BINDING 141 141 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
K
BINDING 187 187 /ligand="ATP"
/ligand_id="ChEBI:CHEBI:30616"
D

Additional information [?]

Size range 291-313 amino acids
Related rules None
Fusion Nter: <Unknown> Cter: None
Comments The ribosomal protein bS6-modifying function does not apply to all RimK family members since some bacteria do not possess a rpsF gene in their genome, or the C-terminal sequence of RpsF is not conserved, or does not end with a glutamate residue.



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