HAMAP annotation rule MF_01569
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Accession MF_01569
Dates 18-AUG-2006 (Created)
2-DEC-2011 (Last updated, Version 12)
Name Pro_tRNA_synth_type1
Scope Bacteria
Triggered by



Identifier
SYP
Protein name
RecName: Full=Proline--tRNA ligase;
EC=6.1.1.15;
AltName: Full=Prolyl-tRNA synthetase;
Short=ProRS;
Gene name
proS


FUNCTION Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction: proline is first activated by ATP to form Pro-AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids such as alanine and cysteine, to avoid such errors it has two additional distinct editing activities against alanine. One activity is designated as 'pretransfer' editing and involves the tRNA(Pro)-independent hydrolysis of activated Ala-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Ala-tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS (By similarity).
CATALYTIC ACTIVITY ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro).
SUBUNIT Homodimer (By similarity).
SUBCELLULAR LOCATION Cytoplasm (By similarity).
DOMAIN Consists of three domains: the N-terminal catalytic domain, the editing domain and the C-terminal anticodon-binding domain (By similarity).
SIMILARITY Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily.


Cytoplasm
Aminoacyl-tRNA synthetase
ATP-binding
Ligase
Nucleotide-binding
Protein biosynthesis


GO:0005524; Molecular function: ATP binding.
GO:0004827; Molecular function: proline-tRNA ligase activity.
GO:0006433; Biological process: prolyl-tRNA aminoacylation.
GO:0005737; Cellular component: cytoplasm.


PROSITE PS50862; AA_TRNA_LIGASE_II; 1;
Pfam PF03129; HGTP_anticodon; 1;
PF00587; tRNA-synt_2b; 1;
PF04073; YbaK; 1;
PRINTS PR01046; TRNASYNTHPRO; 1;
TIGRFAMs TIGR00409; ProS_fam_II; 1;


Size range: 557-620 amino acids
Related Rules: MF_01570 (SYP); MF_01571 (SYP)
Templates: P16659 (SYP_ECOLI); O66690 (SYP_AQUAE): [Recover all]
Fusion: None
Comments: Some species have a second copy of proS in another subfamily (MF_01571).


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