HAMAP

Accession	MF_01588
Dates	16-NOV-2007 (Created) 2-DEC-2011 (Last updated, Version 8)

Data class

Protein

Predictors

HAMAP; MF_01588; [distribution of match scores in UniProtKB];[seed alignment for MF_01588]

Names

DNA_ligase_A

Identifier

DNLJ

Protein name

RecName: Full=DNA ligase; EC=6.5.1.2; AltName: Full=Polydeoxyribonucleotide synthase [NAD+];

Gene name

ligA

FUNCTION: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA (By similarity).

CATALYTIC ACTIVITY: NAD(+) + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + nicotinamide nucleotide + (deoxyribonucleotide)(n+m).

COFACTOR: Magnesium or manganese (By similarity).

SIMILARITY: Belongs to the NAD-dependent DNA ligase family. LigA subfamily.

PROSITE	PS50172; BRCT; 1; trigger=PRU00033; PS01055; DNA_LIGASE_N1; 1; PS01056; DNA_LIGASE_N2; 1;
Pfam	PF00533; BRCT; 1; PF01653; DNA_ligase_aden; 1; PF03120; DNA_ligase_OB; 1; PF03119; DNA_ligase_ZBD; 1;
PIRSF	PIRSF001604; LigA; 1;
TIGRFAMs	TIGR00575; Dnlj; 1;

DNA damage, DNA repair, DNA replication, Ligase, Magnesium, Manganese, Metal-binding, NAD, Zinc.

GO:0003911; Molecular function: DNA ligase (NAD+) activity.
GO:0006259; Biological process: DNA metabolic process.

From: DNLJ_ENTFA (Q837V6)
Key		From		To		Description		Condition		FTGroup
NP_BIND		39		43		NAD (By similarity)		D-x-x-[YF]-D
NP_BIND		88		89		NAD (By similarity)		S-x
ACT_SITE		120		120		N6-AMP-lysine intermediate (By similarity)		K
METAL		409		409		Zinc (By similarity)		C
METAL		412		412		Zinc (By similarity)		C
METAL		427		427		Zinc (By similarity)		C
METAL		432		432		Zinc (By similarity)		C
BINDING (Optional)		118		118		NAD (By similarity)		[ED]
BINDING		141		141		NAD (By similarity)		R
BINDING		175		175		NAD (By similarity)		E
BINDING (Optional)		291		291		NAD (By similarity)		K
BINDING		315		315		NAD (By similarity)		K

Size range:	587-921 amino acids
Related UniRules:	MF_01577 (MDTD (supersedes the current rule))
Template:	O87703 (DNLJ_GEOSE); P15042 (DNLJ_ECOLI); Q9AIU7 (DNLJ_STAAU); P63973 (DNLJ_MYCTU); Q837V6 (DNLJ_ENTFA); Q9ZHI0 (DNLJ_THEFI): [Recover all]
Scope:	Bacteria Archaea
Fusion:	Nter: None; Cter: None
Duplicate:	in ARTCA, CLOAB, DEIDV, DESVV, NOCFA, OPITP, STRCO, STRGG
Plasmid encoded:	in ARTCA, DEIDV