HAMAP rule MF_01588
General rule information
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| Accession | MF_01588 |
| Dates | 16-NOV-2007 (Created)
1-JUN-2023 (Last updated, Version 21) |
| Name | DNA_ligase_A |
| Scope(s) |
Bacteria Archaea |
| Template(s) | O87703 (DNLJ_GEOSE); P15042 (DNLJ_ECOLI); Q9AIU7 (DNLJ_STAAU); P9WNV1 (DNLJ_MYCTU); Q837V6 (DNLJ_ENTFA); Q9ZHI0 (DNLJ_THEFI); [ Recover all ] |
| Triggered by |
HAMAP; MF_01588 (Get profile general information and statistics) |
Propagated annotation
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Identifier, protein and gene names
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| Identifier | DNLJ |
| Protein name | RecName: Full=DNA ligase; EC=6.5.1.2; AltName: Full=Polydeoxyribonucleotide synthase [NAD(+)]; |
| Gene name | Name=ligA; |
Comments
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| FUNCTION | DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double- stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of damaged DNA. |
| CATALYTIC ACTIVITY | Reaction=NAD(+) + (deoxyribonucleotide)n-3'-hydroxyl + 5'-phospho- (deoxyribonucleotide)m = (deoxyribonucleotide)n+m + AMP + beta- nicotinamide D-nucleotide.; EC=6.5.1.2; |
| COFACTOR | Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; |
| SIMILARITY | Belongs to the NAD-dependent DNA ligase family. LigA subfamily. |
Keywords
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Gene Ontology
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| GO:0003911; Molecular function:DNA ligase (NAD+) activity |
| GO:0006259; Biological process:DNA metabolic process |
Cross-references
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| PROSITE | PS50172; BRCT; 1; |
| PROSITE | PS01055; DNA_LIGASE_N1; 1; |
| PROSITE | PS01056; DNA_LIGASE_N2; 1; |
| Pfam | PF00533; BRCT; 1; |
| Pfam | PF01653; DNA_ligase_aden; 1; |
| Pfam | PF03120; DNA_ligase_OB; 1; |
| Pfam | PF03119; DNA_ligase_ZBD; 1; |
| PIRSF | PIRSF001604; LigA; 1; |
| NCBIfam | TIGR00575; Dnlj; 1; |
Features
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| From: DNLJ_ENTFA (Q837V6) | ||||||||||||
| Key | From | To | Description | Tag | Condition | FTGroup | ||||||
| BINDING | 39 | 43 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
D-x-x-[YF]-D | ||||||||
| BINDING | 88 | 89 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
S-x | ||||||||
| ACT_SITE | 120 | 120 | /note="N6-AMP-lysine intermediate" | K | ||||||||
| BINDING | 409 | 409 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | ||||||||
| BINDING | 412 | 412 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | ||||||||
| BINDING | 427 | 427 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | ||||||||
| BINDING | 432 | 432 | /ligand="Zn(2+)" /ligand_id="ChEBI:CHEBI:29105" |
C | ||||||||
| BINDING | 118 | 118 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
[ED] | ||||||||
| BINDING | 141 | 141 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
R | ||||||||
| BINDING | 175 | 175 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
E | ||||||||
| BINDING | 291 | 291 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
K | ||||||||
| BINDING | 315 | 315 | /ligand="NAD(+)" /ligand_id="ChEBI:CHEBI:57540" |
K | ||||||||
Additional information
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| Size range | 587-921 amino acids |
| Related rules |
MF_01577 |
| Fusion | Nter: None Cter: None |